Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText.
Kick-your-searchterm to multiple Engines kick-your-query now !>
A dictionary by aggregated review articles of nephrology, medicine and the life sciences
Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

suck abstract from ncbi


10.1161/CIRCRESAHA.125.326443

http://scihub22266oqcxt.onion/10.1161/CIRCRESAHA.125.326443
suck pdf from google scholar
40590119!ä!40590119

Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=40590119&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215

suck abstract from ncbi


Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534

Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534

Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534

Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\40590119.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117
pmid40590119      Circ+Res 2025 ; ä (ä): ä
Nephropedia Template TP

gab.com Text

Twit Text FOAVip

Twit Text #

English Wikipedia


  • Mitochondrial Calcium Uniporter Regulates ITAM-Dependent Platelet Activation #MMPMID40590119
  • Ajanel A; Andrianova I; Kowalczyk M; Menendez Perez J; Bhatt SR; Portier I; Boone T; Ballard-Kordeliski A; Kosaka Y; Chaudhuri D; Paul DS; Bergmeier W; Denorme F; Campbell RA
  • Circ Res 2025[Jul]; ä (ä): ä PMID40590119show ga
  • BACKGROUND: Platelet activation relies on changes in cytoplasmic calcium flux. However, little is known about the role mitochondrial calcium flux plays in platelet activation. Activation induces release of calcium from intracellular stores, which enters the mitochondrial matrix through the MCU (mitochondrial calcium uniporter) to regulate bioenergetics and reactive oxygen species (ROS) formation, as demonstrated in other cells. However, whether MCU contributes to platelet function is unclear. METHODS: We generated platelet-specific Mcu-deficient mice (Mcu(plt)(-/-)) and compared them to littermate wild-type controls (Mcu(plt+/+)). In vitro approaches assessed mitochondrial calcium flux and platelet activation responses to stimulation of immunoreceptor tyrosine-based activation motif (ITAM) receptors and GPCRs (G protein-coupled receptors). In addition, we examined in vivo hemostasis and thrombosis. We also treated human platelets with MCU inhibitors, and platelet function was assessed. RESULTS: Mcu(plt)(-/)(-) platelets had significantly reduced mitochondrial calcium flux in response to activation of ITAM receptors, whereas mitochondrial calcium flux in response to GPCR activation was unchanged. Platelet aggregation was significantly reduced by ITAM activation in Mcu(plt)(-/)(-) platelets, but GPCR-induced aggregation was unchanged. Similar findings were observed when MCU was inhibited in human platelets. In vivo, Mcu(plt)(-/)(-) mice had reduced arterial thrombosis and less ischemic stroke brain injury. Hemostasis was mildly altered in Mcu(plt)(-/)(-) mice. Mechanistically, mitochondrial ROS generation was significantly reduced in Mcu(plt)(-/)(-) platelets compared with Mcu(plt+/+) platelets after ITAM-dependent activation, but not GPCR activation. Reduced mitochondrial ROS was associated with decreased ITAM signaling based on p-Syk (phospho-spleen tyrosine kinase) and p-PLCgamma2 (phospho-phospholipase C-gamma 2) in Mcu(plt)(-/)(-) platelets. Inhibiting mitochondrial ROS decreased aggregation as well as downstream ITAM signaling in Mcu(plt+/+) platelets. Conversely, treating Mcu(plt)(-/)(-) platelets with MitoParaquat to induce mitochondrial ROS increased platelet ITAM-dependent aggregation and signaling. CONCLUSIONS: Our data support a role for mitochondrial calcium flux in regulating ITAM-dependent platelet activation through the generation of mitochondrial ROS.
  • ä


  • DeepDyve
  • Pubget Overpricing
  • suck abstract from ncbi

    Linkout box