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10.21769/bioprotoc.1508 http://scihub22266oqcxt.onion/10.21769/bioprotoc.1508 C4829118!4829118 !27081667     free     free     free Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27081667 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Bio+Protoc 2015 ; 5 (12 ): ä Nephropedia Template TP {{tp|p=27081667 |t=2015. Mismatched Primer Extension Assays |pdf=|usr=}}{{27081667 }} gab.com Text Mismatched Primer Extension Assays JO: Bio Protoc http://www.kidney.de/pget.php?&tw=1&pmid=27081667 #FOAvip Steady state kinetic assays have been a reliable way to estimate fidelity of several polymerases (Menendez-Arias, 2009; Rezende and Prasad, 2004; Svarovskaia et al., 2003). The ability to analyze the extension of primers with specific mismatches at the 3' end is a major strength of the mismatched primer extension assays. Recently, we used the mismatched primer extension assays to show that the fidelity of HIV RT increases dramatically when concentration of Mg(2+) is reduced to a physiologically relevant concentration (~0.25 mM) (Achuthan et al., 2014). Here, we describe in detail how to perform the mismatched primer extension assay to measure the standard extension efficiency using human immunodeficiency virus reverse transcriptase (HIV RT) at 2 mM Mg(2+). The relative fidelity of the polymerase can then be estimated using the standard extension efficiency. The assay described here is based on the method published in Mendelman et al. (1990). Twit Text FOAVip Mismatched Primer Extension Assays ????Bio Protoc http://www.kidney.de/pget.php?&tw=1&pmid=27081667 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27081667 #FOAvip English Wikipedia <ref>{{Cite pmid|27081667 }}</ref> Mismatched Primer Extension Assays #MMPMID27081667 Achuthan V ; DeStefano JJ Bio Protoc 2015[Jun]; 5 (12 ): ä PMID27081667 show ga Steady state kinetic assays have been a reliable way to estimate fidelity of several polymerases (Menendez-Arias, 2009; Rezende and Prasad, 2004; Svarovskaia et al., 2003). The ability to analyze the extension of primers with specific mismatches at the 3' end is a major strength of the mismatched primer extension assays. Recently, we used the mismatched primer extension assays to show that the fidelity of HIV RT increases dramatically when concentration of Mg(2+) is reduced to a physiologically relevant concentration (~0.25 mM) (Achuthan et al., 2014). Here, we describe in detail how to perform the mismatched primer extension assay to measure the standard extension efficiency using human immunodeficiency virus reverse transcriptase (HIV RT) at 2 mM Mg(2+). The relative fidelity of the polymerase can then be estimated using the standard extension efficiency. The assay described here is based on the method published in Mendelman et al. (1990). äMismatched Primer Extension Assays (epmc).pdf DeepDyve Pubget Overpricing