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Microfluidic chambers using fluid walls for cell biology
#MMPMID29895687
Soitu C
; Feuerborn A
; Tan AN
; Walker H
; Walsh PA
; Castrejón-Pita AA
; Cook PR
; Walsh EJ
Proc Natl Acad Sci U S A
2018[Jun]; 115
(26
): E5926-E5933
PMID29895687
show ga
Many proofs of concept have demonstrated the potential of microfluidics in cell
biology. However, the technology remains inaccessible to many biologists, as it
often requires complex manufacturing facilities (such as soft lithography) and
uses materials foreign to cell biology (such as polydimethylsiloxane). Here, we
present a method for creating microfluidic environments by simply reshaping
fluids on a substrate. For applications in cell biology, we use cell media on a
virgin Petri dish overlaid with an immiscible fluorocarbon. A
hydrophobic/fluorophilic stylus then reshapes the media into any pattern by
creating liquid walls of fluorocarbon. Microfluidic arrangements suitable for
cell culture are made in minutes using materials familiar to biologists. The
versatility of the method is demonstrated by creating analogs of a common
platform in cell biology, the microtiter plate. Using this vehicle, we
demonstrate many manipulations required for cell culture and downstream analysis,
including feeding, replating, cloning, cryopreservation, lysis plus RT-PCR,
transfection plus genome editing, and fixation plus immunolabeling (when fluid
walls are reconfigured during use). We also show that mammalian cells grow and
respond to stimuli normally, and worm eggs develop into adults. This simple
approach provides biologists with an entrée into microfluidics.
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