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10.1038/nrg3198

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suck abstract from ncbi


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pmid22510765
      Nat+Rev+Genet 2012 ; 13 (5 ): 358-69
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  • MicroRNA profiling: approaches and considerations #MMPMID22510765
  • Pritchard CC ; Cheng HH ; Tewari M
  • Nat Rev Genet 2012[Apr]; 13 (5 ): 358-69 PMID22510765 show ga
  • MicroRNAs (miRNAs) are small RNAs that post-transcriptionally regulate the expression of thousands of genes in a broad range of organisms in both normal physiological contexts and in disease contexts. miRNA expression profiling is gaining popularity because miRNAs, as key regulators in gene expression networks, can influence many biological processes and also show promise as biomarkers for disease. Technological advances have spawned a multitude of platforms for miRNA profiling, and an understanding of the strengths and pitfalls of different approaches can aid in their effective use. Here, we review the major considerations for carrying out and interpreting results of miRNA-profiling studies.
  • |*Gene Expression Regulation [MESH]
  • |Animals [MESH]
  • |Base Sequence [MESH]
  • |Databases, Genetic [MESH]
  • |Gene Expression Profiling/*methods [MESH]
  • |High-Throughput Nucleotide Sequencing [MESH]
  • |MicroRNAs/analysis/genetics/*metabolism [MESH]
  • |Reverse Transcriptase Polymerase Chain Reaction [MESH]


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