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2012 ; 13
(5
): 358-69
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MicroRNA profiling: approaches and considerations
#MMPMID22510765
Pritchard CC
; Cheng HH
; Tewari M
Nat Rev Genet
2012[Apr]; 13
(5
): 358-69
PMID22510765
show ga
MicroRNAs (miRNAs) are small RNAs that post-transcriptionally regulate the
expression of thousands of genes in a broad range of organisms in both normal
physiological contexts and in disease contexts. miRNA expression profiling is
gaining popularity because miRNAs, as key regulators in gene expression networks,
can influence many biological processes and also show promise as biomarkers for
disease. Technological advances have spawned a multitude of platforms for miRNA
profiling, and an understanding of the strengths and pitfalls of different
approaches can aid in their effective use. Here, we review the major
considerations for carrying out and interpreting results of miRNA-profiling
studies.