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Methods for Studying Autophagy Within the Tumor Microenvironment
#MMPMID27325266
Murugan S
; Amaravadi RK
Adv Exp Med Biol
2016[]; 899
(?): 145-66
PMID27325266
show ga
Defective autophagy has been linked with many pathologies, including cancer,
diabetes, infectious disease, myopathies, heart, liver, lung, and
neurodegenerative disease. Autophagy has therefore become an important target in
drug discovery. Recent advances have identified new ways to monitor autophagy in
vitro and in vivo. Many assays rely on visualizing autophagy-related
intracellular markers such as microtubule-associated protein light chain 3 (LC3)
II, which have posed issues with in vivo and clinical translation of the in vitro
assays. Here, we present an overview of current in vitro and in vivo
methodologies to measure autophagy with a special focus on the tumor
microenvironment.