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Melanoma educates mesenchymal stromal cells towards vasculogenic mimicry
#MMPMID27313776
Vartanian A
; Karshieva S
; Dombrovsky V
; Belyavsky A
Oncol Lett
2016[Jun]; 11
(6
): 4264-4268
PMID27313776
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Accumulating evidence suggests that mesenchymal stromal cells (MSCs) are
recruited to the tumor, and promote tumor development and growth. The present
study was performed to investigate the communication between aggressive melanoma
and MSCs in vasculogenic mimicry (VM). Normal human MSCs plated on Matrigel were
unable to form capillary-like structures (CLSs). By contrast, MSCs co-cultured
with aggressive melanoma cell lines, namely, Mel Cher, Mel Kor and Mel P,
generated CLSs. Significantly, MSCs co-cultured with poorly aggressive melanoma
cells, namely, Mel Me, failed to form CLSs. To identify factors responsible for
VM, the effects of vascular endothelial growth factor A (VEGFA), pro-epidermal
growth factor, basic fibroblast growth factor and stromal cell-derived factor 1?
on the formation of CLSs by MSCs were tested. VM was induced by the addition of
VEGFA, whereas other cytokines were inefficient. To confirm the hypothesis that
aggressive tumor cells can increase the vasculogenic ability of MSCs, a standard
B16/F10 mouse melanoma test system was used. MSCs isolated from the adipose
tissues of C57BL/6 mice with melanoma formed a vascular-like network on Matrigel,
whereas MSCs from healthy mice failed to form such structures. This study
provides the first direct evidence that melanoma tumors educate MSCs to engage in
VM. The education may occur distantly. These findings offer promise for novel
therapeutic directions in the treatment of metastatic melanoma.
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