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2016 ; 64
(3
): 443-454
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Mediator Undergoes a Compositional Change during Transcriptional Activation
#MMPMID27773675
Petrenko N
; Jin Y
; Wong KH
; Struhl K
Mol Cell
2016[Nov]; 64
(3
): 443-454
PMID27773675
show ga
Mediator is a transcriptional co-activator recruited to enhancers by DNA-binding
activators, and it also interacts with RNA polymerase (Pol) II as part of
the preinitiation complex (PIC). We demonstrate that a single Mediator complex
associates with the enhancer and core promoter in vivo, indicating that it can
physically bridge these transcriptional elements. However, the Mediator kinase
module associates strongly with the enhancer, but not with the core promoter, and
it dissociates from the enhancer upon depletion of the TFIIH kinase. Severing the
kinase module from Mediator by removing the connecting subunit Med13 does not
affect Mediator association at the core promoter but increases occupancy at
enhancers. Thus, Mediator undergoes a compositional change in which the kinase
module, recruited via Mediator to the enhancer, dissociates from Mediator to
permit association with Pol II and the PIC. As such, Mediator acts as a dynamic
bridge between the enhancer and core promoter.
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