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2016 ; 428
(9 Pt A
): 1700-13
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Mechanistically Dissecting Autophagy: Insights from In Vitro Reconstitution
#MMPMID26946034
Brier LW
; Zhang M
; Ge L
J Mol Biol
2016[May]; 428
(9 Pt A
): 1700-13
PMID26946034
show ga
Autophagy is a fundamental cellular mechanism responsible for bulk turnover of
cytoplasmic components. It is broadly related to many cellular activities,
physiological processes, and pathological conditions. Autophagy entails a
spatiotemporal interaction between cytosolic factors and membranes that are
remodeled to encapsulate autophagic cargo within an autophagosome. Although
majority of the factors [autophagy-related gene (Atg) proteins] involved in
autophagy have been identified by genetic studies, the mechanism accounting for
how these factors act upon the membrane to remodel it and efficiently recruit
cargo for degradation is unclear. In vitro reconstitution of several different
aspects of autophagy has provided important insights into the understanding of
the mechanistic details underlying autophagic membrane remodeling and cargo
recruitment. Here, we highlight these efforts toward studying autophagy through
in vitro approaches.