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2010 ; 1203
(ä): 120-5
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Mechanisms of oxidant generation by catalase
#MMPMID20716293
Heck DE
; Shakarjian M
; Kim HD
; Laskin JD
; Vetrano AM
Ann N Y Acad Sci
2010[Aug]; 1203
(ä): 120-5
PMID20716293
show ga
The enzyme catalase converts solar radiation into reactive oxidant species (ROS).
In this study, we report that several bacterial catalases (hydroperoxidases, HP),
including Escherichia coli HP-I and HP-II also generate reactive oxidants in
response to ultraviolet B light (UVB). HP-I and HP-II are identical except for
the presence of NADPH. We found that only one of the catalases, HPI, produces
oxidants in response to UVB light, indicating a potential role for the nucleotide
in ROS production. This prompts us to speculate that NADPH may act as a cofactor
regulating ROS generation by mammalian catalases. Structural analysis of the
NADPH domains of several mammalian catalases revealed that the nucleotide is
bound in a constrained conformation and that UVB irradiation induces NADPH
oxidation and positional changes. Biochemical and kinetic analysis indicate that
ROS formation by the enzyme is enhanced by oxidation of the cofactor.
Conformational changes following absorption of UVB light by catalase NADPH have
the potential to facilitate ROS production by the enzyme.
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