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10.1016/j.cell.2014.05.038 http://scihub22266oqcxt.onion/10.1016/j.cell.2014.05.038 C4105854!4105854 !25036631     free     free     free Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25036631 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Cell 2014 ; 158 (2 ): 314-326 Nephropedia Template TP {{tp|p=25036631 |t=2014. Mechanism of transcriptional bursting in bacteria |pdf=|usr=}}{{25036631 }} gab.com Text Mechanism of transcriptional bursting in bacteria JO: Cell http://www.kidney.de/pget.php?&tw=1&pmid=25036631 #FOAvip Transcription of highly expressed genes has been shown to occur in stochastic bursts. But the origin of such ubiquitous phenomenon has not been understood. Here, we present the mechanism in bacteria. We developed a high-throughput, in vitro, single-molecule assay to follow transcription on individual DNA templates in real time. We showed that positive supercoiling buildup on a DNA segment by transcription slows down transcription elongation and eventually stops transcription initiation. Transcription can be resumed upon gyrase binding to the DNA segment. Furthermore, using single-cell mRNA counting fluorescence in situ hybridization (FISH), we found that duty cycles of transcriptional bursting depend on the intracellular gyrase concentration. Together, these findings prove that transcriptional bursting of highly expressed genes in bacteria is primarily caused by reversible gyrase dissociation from and rebinding to a DNA segment, changing the supercoiling level of the segment. Twit Text FOAVip Mechanism of transcriptional bursting in bacteria ????Cell http://www.kidney.de/pget.php?&tw=1&pmid=25036631 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25036631 #FOAvip English Wikipedia <ref>{{Cite pmid|25036631 }}</ref> Mechanism of transcriptional bursting in bacteria #MMPMID25036631 Chong S ; Chen C ; Ge H ; Xie XS Cell 2014[Jul]; 158 (2 ): 314-326 PMID25036631 show ga Transcription of highly expressed genes has been shown to occur in stochastic bursts. But the origin of such ubiquitous phenomenon has not been understood. Here, we present the mechanism in bacteria. We developed a high-throughput, in vitro, single-molecule assay to follow transcription on individual DNA templates in real time. We showed that positive supercoiling buildup on a DNA segment by transcription slows down transcription elongation and eventually stops transcription initiation. Transcription can be resumed upon gyrase binding to the DNA segment. Furthermore, using single-cell mRNA counting fluorescence in situ hybridization (FISH), we found that duty cycles of transcriptional bursting depend on the intracellular gyrase concentration. Together, these findings prove that transcriptional bursting of highly expressed genes in bacteria is primarily caused by reversible gyrase dissociation from and rebinding to a DNA segment, changing the supercoiling level of the segment. |*Transcription, Genetic [MESH] |DNA Gyrase/metabolism [MESH] |DNA, Superhelical/genetics [MESH] |Escherichia coli/*genetics [MESH] |In Situ Hybridization, Fluorescence [MESH] |Models, Genetic [MESH] |Promoter Regions, Genetic [MESH] |Transcription Elongation, Genetic [MESH]Mechanism of transcriptional bursting in bacteria (epmc).pdf DeepDyve Pubget Overpricing