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2017 ; 28
(5
): 645-660
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Mechanism of nuclear movements in a multinucleated cell
#MMPMID28077618
Gibeaux R
; Politi AZ
; Philippsen P
; Nédélec F
Mol Biol Cell
2017[Mar]; 28
(5
): 645-660
PMID28077618
show ga
Multinucleated cells are important in many organisms, but the mechanisms
governing the movements of nuclei sharing a common cytoplasm are not understood.
In the hyphae of the plant pathogenic fungus Ashbya gossypii, nuclei move back
and forth, occasionally bypassing each other, preventing the formation of nuclear
clusters. This is essential for genetic stability. These movements depend on
cytoplasmic microtubules emanating from the nuclei that are pulled by dynein
motors anchored at the cortex. Using three-dimensional stochastic simulations
with parameters constrained by the literature, we predict the cortical anchor
density from the characteristics of nuclear movements. The model accounts for the
complex nuclear movements seen in vivo, using a minimal set of experimentally
determined ingredients. Of interest, these ingredients power the oscillations of
the anaphase spindle in budding yeast, but in A. gossypii, this system is not
restricted to a specific nuclear cycle stage, possibly as a result of adaptation
to hyphal growth and multinuclearity.