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2014 ; 107
(11
): 2693-9
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Mechanics of individual keratin bundles in living cells
#MMPMID25468348
Nolting JF
; Möbius W
; Köster S
Biophys J
2014[Dec]; 107
(11
): 2693-9
PMID25468348
show ga
Along with microtubules and microfilaments, intermediate filaments are a major
component of the eukaryotic cytoskeleton and play a key role in cell mechanics.
In cells, keratin intermediate filaments form networks of bundles that are
sparser in structure and have lower connectivity than, for example, actin
networks. Because of this, bending and buckling play an important role in these
networks. Buckling events, which occur due to compressive intracellular forces
and cross-talk between the keratin network and other cytoskeletal components, are
measured here in situ. By applying a mechanical model for the bundled filaments,
we can access the mechanical properties of both the keratin bundles themselves
and the surrounding cytosol. Bundling is characterized by a coupling parameter
that describes the strength of the linkage between the individual filaments
within a bundle. Our findings suggest that coupling between the filaments is
mostly complete, although it becomes weaker for thicker bundles, with some
relative movement allowed.
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