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10.1126/sciadv.1602536 http://scihub22266oqcxt.onion/10.1126/sciadv.1602536 C5470826!5470826 !28630905     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28630905 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Sci+Adv 2017 ; 3 (6 ): e1602536 Nephropedia Template TP {{tp|p=28630905 |t=2017. Mechanical deformation induces depolarization of neutrophils |pdf=|usr=}}{{28630905 }} gab.com Text Mechanical deformation induces depolarization of neutrophils JO: Sci Adv http://www.kidney.de/pget.php?&tw=1&pmid=28630905 #FOAvip The transition of neutrophils from a resting state to a primed state is an essential requirement for their function as competent immune cells. This transition can be caused not only by chemical signals but also by mechanical perturbation. After cessation of either, these cells gradually revert to a quiescent state over 40 to 120 min. We use two biophysical tools, an optical stretcher and a novel microcirculation mimetic, to effect physiologically relevant mechanical deformations of single nonadherent human neutrophils. We establish quantitative morphological analysis and mechanical phenotyping as label-free markers of neutrophil priming. We show that continued mechanical deformation of primed cells can cause active depolarization, which occurs two orders of magnitude faster than by spontaneous depriming. This work provides a cellular-level mechanism that potentially explains recent clinical studies demonstrating the potential importance, and physiological role, of neutrophil depriming in vivo and the pathophysiological implications when this deactivation is impaired, especially in disorders such as acute lung injury. Twit Text FOAVip Mechanical deformation induces depolarization of neutrophils ????Sci Adv http://www.kidney.de/pget.php?&tw=1&pmid=28630905 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28630905 #FOAvip English Wikipedia <ref>{{Cite pmid|28630905 }}</ref> Mechanical deformation induces depolarization of neutrophils #MMPMID28630905 Ekpenyong AE ; Toepfner N ; Fiddler C ; Herbig M ; Li W ; Cojoc G ; Summers C ; Guck J ; Chilvers ER Sci Adv 2017[Jun]; 3 (6 ): e1602536 PMID28630905 show ga The transition of neutrophils from a resting state to a primed state is an essential requirement for their function as competent immune cells. This transition can be caused not only by chemical signals but also by mechanical perturbation. After cessation of either, these cells gradually revert to a quiescent state over 40 to 120 min. We use two biophysical tools, an optical stretcher and a novel microcirculation mimetic, to effect physiologically relevant mechanical deformations of single nonadherent human neutrophils. We establish quantitative morphological analysis and mechanical phenotyping as label-free markers of neutrophil priming. We show that continued mechanical deformation of primed cells can cause active depolarization, which occurs two orders of magnitude faster than by spontaneous depriming. This work provides a cellular-level mechanism that potentially explains recent clinical studies demonstrating the potential importance, and physiological role, of neutrophil depriming in vivo and the pathophysiological implications when this deactivation is impaired, especially in disorders such as acute lung injury. |*Mechanical Phenomena [MESH] |Cell Shape [MESH] |Humans [MESH] |Neutrophil Activation/immunology [MESH] |Neutrophil Infiltration [MESH]Mechanical deformation induces depolarization of neutrophils (epmc).pdf DeepDyve Pubget Overpricing