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2016 ; 1458
(ä): 71-85
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Measuring Vascular Permeability In Vivo
#MMPMID27581015
Meijer EF
; Baish JW
; Padera TP
; Fukumura D
Methods Mol Biol
2016[]; 1458
(ä): 71-85
PMID27581015
show ga
Over the past decades, in vivo vascular permeability measurements have provided
significant insight into vascular functions in physiological and
pathophysiological conditions such as the response to pro- and anti-angiogenic
signaling, abnormality of tumor vasculature and its normalization, and delivery
and efficacy of therapeutic agents. Different approaches for vascular
permeability measurements have been established. Here, we describe and discuss a
conventional 2D imaging method to measure vascular permeability, which was
originally documented by Gerlowski and Jain in 1986 (Microvasc Res 31:288-305,
1986) and further developed by Yuan et al. in the early 1990s (Microvasc Res
45:269-289, 1993; Cancer Res 54:352-3356, 1994), and our recently developed 3D
imaging method, which advances the approach originally described by Brown et al.
in 2001 (Nat Med 7:864-868, 2001).