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2017 ; 427
(ä): 100-104
Nephropedia Template TP
Tefft BJ
; Uthamaraj S
; Harburn JJ
; Hlinomaz O
; Lerman A
; Dragomir-Daescu D
; Sandhu GS
J Magn Magn Mater
2017[Apr]; 427
(ä): 100-104
PMID28286359
show ga
Emerging nanotechnologies have enabled the use of magnetic forces to guide the
movement of magnetically-labeled cells, drugs, and other therapeutic agents.
Endothelial cells labeled with superparamagnetic iron oxide nanoparticles (SPION)
have previously been captured on the surface of magnetizable 2205 duplex
stainless steel stents in a porcine coronary implantation model. Recently, we
have coated these stents with electrospun polyurethane nanofibers to fabricate
prototype stent-grafts. Facilitated endothelialization may help improve the
healing of arteries treated with stent-grafts, reduce the risk of thrombosis and
restenosis, and enable small-caliber applications. When placed in a SPION-labeled
endothelial cell suspension in the presence of an external magnetic field,
magnetized stent-grafts successfully captured cells to the surface regions
adjacent to the stent struts. Implantation within the coronary circulation of
pigs (n=13) followed immediately by SPION-labeled autologous endothelial cell
delivery resulted in widely patent devices with a thin, uniform neointima and no
signs of thrombosis or inflammation at 7 days. Furthermore, the magnetized
stent-grafts successfully captured and retained SPION-labeled endothelial cells
to select regions adjacent to stent struts and between stent struts, whereas the
non-magnetized control stent-grafts did not. Early results with these prototype
devices are encouraging and further refinements will be necessary in order to
achieve more uniform cell capture and complete endothelialization. Once
optimized, this approach may lead to more rapid and complete healing of vascular
stent-grafts with a concomitant improvement in long-term device performance.