Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27600077
.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Microarrays+(Basel)
2016 ; 5
(2
): ä Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Living Cell Microarrays: An Overview of Concepts
#MMPMID27600077
Jonczyk R
; Kurth T
; Lavrentieva A
; Walter JG
; Scheper T
; Stahl F
Microarrays (Basel)
2016[May]; 5
(2
): ä PMID27600077
show ga
Living cell microarrays are a highly efficient cellular screening system. Due to
the low number of cells required per spot, cell microarrays enable the use of
primary and stem cells and provide resolution close to the single-cell level.
Apart from a variety of conventional static designs, microfluidic microarray
systems have also been established. An alternative format is a microarray
consisting of three-dimensional cell constructs ranging from cell spheroids to
cells encapsulated in hydrogel. These systems provide an in vivo-like
microenvironment and are preferably used for the investigation of cellular
physiology, cytotoxicity, and drug screening. Thus, many different high-tech
microarray platforms are currently available. Disadvantages of many systems
include their high cost, the requirement of specialized equipment for their
manufacture, and the poor comparability of results between different platforms.
In this article, we provide an overview of static, microfluidic, and 3D cell
microarrays. In addition, we describe a simple method for the printing of living
cell microarrays on modified microscope glass slides using standard DNA
microarray equipment available in most laboratories. Applications in research and
diagnostics are discussed, e.g., the selective and sensitive detection of
biomarkers. Finally, we highlight current limitations and the future prospects of
living cell microarrays.
free
free
free
