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2014 ; 307
(10
): F1162-8
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Live nephron imaging by MRI
#MMPMID25186296
Qian C
; Yu X
; Pothayee N
; Dodd S
; Bouraoud N
; Star R
; Bennett K
; Koretsky A
Am J Physiol Renal Physiol
2014[Nov]; 307
(10
): F1162-8
PMID25186296
show ga
The local sensitivity of MRI can be improved with small MR detectors placed close
to regions of interest. However, to maintain such sensitivity advantage, local
detectors normally need to communicate with the external amplifier through cable
connections, which prevent the use of local detectors as implantable devices.
Recently, an integrated wireless amplifier was developed that can efficiently
amplify and broadcast locally detected signals, so that the local sensitivity was
enhanced without the need for cable connections. This integrated detector enabled
the live imaging of individual glomeruli using negative contrast introduced by
cationized ferritin, and the live imaging of renal tubules using positive
contrast introduced by gadopentetate dimeglumine. Here, we utilized the high
blood flow to image individual glomeruli as hyperintense regions without any
contrast agent. These hyperintense regions were identified for pixels with signal
intensities higher than the local average. Addition of Mn(2+) allowed the
simultaneous detection of both glomeruli and renal tubules: Mn(2+) was primarily
reabsorbed by renal tubules, which would be distinguished from glomeruli due to
higher enhancement in T1-weighted MRI. Dynamic studies of Mn(2+) absorption
confirmed the differential absorption affinity of glomeruli and renal tubules,
potentially enabling the in vivo observation of nephron function.
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