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2018 ; 1649
(ä): 349-357
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Live Imaging of mRNA Synthesis in Drosophila
#MMPMID29130209
Garcia HG
; Gregor T
Methods Mol Biol
2018[]; 1649
(ä): 349-357
PMID29130209
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mRNA synthesis is one of the earliest readouts of the activity of a transcribed
gene, which is of particular interest in the context of metazoan cell fate
specification. These processes are intrinsically dynamic and stochastic, which
makes in vivo single-cell measurements inevitable. Here, we present the
application of a technology that has been widely used in single celled organisms
to measure transcriptional activity in developing embryos of the fruit fly
Drosophila melanogaster. The method allows for quantification of instantaneous
polymerase occupancy of active gene loci and thereby enables the development and
testing of models of gene regulation in development.