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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 J+Clin+Oncol
2014 ; 32
(6
): 579-86
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Liquid biopsies: genotyping circulating tumor DNA
#MMPMID24449238
Diaz LA Jr
; Bardelli A
J Clin Oncol
2014[Feb]; 32
(6
): 579-86
PMID24449238
show ga
Genotyping tumor tissue in search of somatic genetic alterations for actionable
information has become routine practice in clinical oncology. Although these
sequence alterations are highly informative, sampling tumor tissue has
significant inherent limitations; tumor tissue is a single snapshot in time, is
subject to selection bias resulting from tumor heterogeneity, and can be
difficult to obtain. Cell-free fragments of DNA are shed into the bloodstream by
cells undergoing apoptosis or necrosis, and the load of circulating cell-free DNA
(cfDNA) correlates with tumor staging and prognosis. Moreover, recent advances in
the sensitivity and accuracy of DNA analysis have allowed for genotyping of cfDNA
for somatic genomic alterations found in tumors. The ability to detect and
quantify tumor mutations has proven effective in tracking tumor dynamics in real
time as well as serving as a liquid biopsy that can be used for a variety of
clinical and investigational applications not previously possible.
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