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10.1152/ajpgi.00047.2016 http://scihub22266oqcxt.onion/10.1152/ajpgi.00047.2016 C5075999!5075999 !27514475     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27514475 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Am+J+Physiol+Gastrointest+Liver+Physiol 2016 ; 311 (3 ): G548-60 Nephropedia Template TP {{tp|p=27514475 |t=2016. Legumain is activated in macrophages during pancreatitis |pdf=|usr=}}{{27514475 }} gab.com Text Legumain is activated in macrophages during pancreatitis JO: Am J Physiol Gastrointest Liver Physiol http://www.kidney.de/pget.php?&tw=1&pmid=27514475 #FOAvip Pancreatitis is an inflammatory disease of the pancreas characterized by dysregulated activity of digestive enzymes, necrosis, immune infiltration, and pain. Repeated incidence of pancreatitis is an important risk factor for pancreatic cancer. Legumain, a lysosomal cysteine protease, has been linked to inflammatory diseases such as atherosclerosis, stroke, and cancer. Until now, legumain activation has not been studied during pancreatitis. We used a fluorescently quenched activity-based probe to assess legumain activation during caerulein-induced pancreatitis in mice. We detected activated legumain by ex vivo imaging, confocal microscopy, and gel electrophoresis. Compared with healthy controls, legumain activity in the pancreas of caerulein-treated mice was increased in a time-dependent manner. Legumain was localized to CD68(+) macrophages and was not active in pancreatic acinar cells. Using a small-molecule inhibitor of legumain, we found that this protease is not essential for the initiation of pancreatitis. However, it may serve as a biomarker of disease, since patients with chronic pancreatitis show strongly increased legumain expression in macrophages. Moreover, the occurrence of legumain-expressing macrophages in regions of acinar-to-ductal metaplasia suggests that this protease may influence reprogramming events that lead to inflammation-induced pancreatic cancer. Twit Text FOAVip Legumain is activated in macrophages during pancreatitis ????Am J Physiol Gastrointest Liver Physiol http://www.kidney.de/pget.php?&tw=1&pmid=27514475 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27514475 #FOAvip English Wikipedia <ref>{{Cite pmid|27514475 }}</ref> Legumain is activated in macrophages during pancreatitis #MMPMID27514475 Edgington-Mitchell LE ; Wartmann T ; Fleming AK ; Gocheva V ; van der Linden WA ; Withana NP ; Verdoes M ; Aurelio L ; Edgington-Mitchell D ; Lieu T ; Parker BS ; Graham B ; Reinheckel T ; Furness JB ; Joyce JA ; Storz P ; Halangk W ; Bogyo M ; Bunnett NW Am J Physiol Gastrointest Liver Physiol 2016[Sep]; 311 (3 ): G548-60 PMID27514475 show ga Pancreatitis is an inflammatory disease of the pancreas characterized by dysregulated activity of digestive enzymes, necrosis, immune infiltration, and pain. Repeated incidence of pancreatitis is an important risk factor for pancreatic cancer. Legumain, a lysosomal cysteine protease, has been linked to inflammatory diseases such as atherosclerosis, stroke, and cancer. Until now, legumain activation has not been studied during pancreatitis. We used a fluorescently quenched activity-based probe to assess legumain activation during caerulein-induced pancreatitis in mice. We detected activated legumain by ex vivo imaging, confocal microscopy, and gel electrophoresis. Compared with healthy controls, legumain activity in the pancreas of caerulein-treated mice was increased in a time-dependent manner. Legumain was localized to CD68(+) macrophages and was not active in pancreatic acinar cells. Using a small-molecule inhibitor of legumain, we found that this protease is not essential for the initiation of pancreatitis. However, it may serve as a biomarker of disease, since patients with chronic pancreatitis show strongly increased legumain expression in macrophages. Moreover, the occurrence of legumain-expressing macrophages in regions of acinar-to-ductal metaplasia suggests that this protease may influence reprogramming events that lead to inflammation-induced pancreatic cancer. |Animals [MESH] |Ceruletide/toxicity [MESH] |Cysteine Endopeptidases/genetics/*metabolism [MESH] |Enzyme Inhibitors/pharmacology [MESH] |Female [MESH] |Gene Expression Regulation, Enzymologic [MESH] |Macrophages/*enzymology/metabolism [MESH] |Mice [MESH] |Mice, Inbred C57BL [MESH] |Mice, Knockout [MESH]Legumain is activated in macrophages during pancreatitis (epmc).pdf DeepDyve Pubget Overpricing