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2015 ; 2015
(ä): 282806
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Lactoferrin Induces Osteoblast Growth through IGF-1R
#MMPMID26290662
Hou JM
; Chen EY
; Lin F
; Lin QM
; Xue Y
; Lan XH
; Wu M
Int J Endocrinol
2015[]; 2015
(ä): 282806
PMID26290662
show ga
Objectives. To investigate the role of the IGF-1R by which lactoferrin induces
osteoblast growth. Methods. Osteoblast received 5?d lactoferrin intervention at a
concentration of 0.1, 1, 10, 100, and 1000??g/mL, and the IGF-1 and IGF-1R were
detected using RT-PCR and western blot. The osteoblast into the control,
100??g/mL lactoferrin, Neo-scramble (NS, empty vector), NS + 100??g/mL
lactoferrin, shIGF-1R and shIGF-1R + 100??g/mL lactoferrin group. We test the
apoptosis and proliferation and the level of PI3K and RAS in osteoblasts after
5?d intervention. Results. (1) 1, 10, 100, and 1000??g/mL lactoferrin induced the
expression of IGF-1 mRNA and protein. 10??g/mL and 100??g/mL lactoferrin induced
the expression of IGF-1R mRNA and protein. (2) Lactoferrin (100??g/mL) induced
osteoblast proliferation while inhibiting apoptosis. Osteoblasts with silenced
IGF-1R exhibited decreased proliferation but increased apoptosis. MMT staining
and flow cytometry both indicated that there was no significant difference
between the shIGF-1R group and the shIGF-1R + 100??g/mL lactoferrin group. (3)
Lactoferrin (100??g/mL) induced PI3K and RAS phosphorylation and silence of
IGF-1R resulted in decreased p-PI3K and p-RAS expression. Lactoferrin-treated
shIGF-1R cells showed significantly higher level of p-PI3K and p-RAS when
compared with shIGF-1R. Conclusion. Lactoferrin induced IGF-1/IGF-1R in a
concentration-dependent manner. Lactoferrin promoted osteoblast proliferation
while inhibiting apoptosis through IGF-1R. Lactoferrin activated PI3K and RAS
phosphorylation via an IGF-1R independent pathway.
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