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2017 ; 14
(7
): 831-834
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Issues in current microRNA target identification methods
#MMPMID28430005
Seitz H
RNA Biol
2017[Jul]; 14
(7
): 831-834
PMID28430005
show ga
microRNAs are currently believed to control a large diversity of physiologic
processes, through the collective repression of thousands of target genes. Both
experimental and computational analyses indeed suggest that each microRNA
regulates tens or hundreds of genes. But some observations suggest that the
phenotypic consequences of many published miRNA/mRNA interactions are dubious.
For example, the reported amplitude of miRNA-guided repression is very small,
while biologic processes tend to be robust to small changes in gene expression.
We recently showed, on one particular miRNA, that for most predicted targets,
miRNA-guided repression is even smaller than inter-individual variability among
wild-type specimens. We also put forward several sources of computational false
positives. These issues are generally neglected by the scientific community,
probably resulting in the frequent publication of irreproducible or
misinterpreted results regarding microRNA function. We propose novel types of
analyses, easily accessible to the community, that could help improve microRNA
target identification.
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