Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26794529
.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Methods+Cell+Biol
2016 ; 131
(ä): 467-86
Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Isolation and analyses of axonal ribonucleoprotein complexes
#MMPMID26794529
Doron-Mandel E
; Alber S
; Oses JA
; Medzihradszky KF
; Burlingame AL
; Fainzilber M
; Twiss JL
; Lee SJ
Methods Cell Biol
2016[]; 131
(ä): 467-86
PMID26794529
show ga
Cytoskeleton-dependent RNA transport and local translation in axons are gaining
increased attention as key processes in the maintenance and functioning of
neurons. Specific axonal transcripts have been found to play roles in many
aspects of axonal physiology including axon guidance, axon survival, axon to soma
communication, injury response and regeneration. This axonal transcriptome
requires long-range transport that is achieved by motor proteins carrying
transcripts as messenger ribonucleoprotein (mRNP) complexes along microtubules.
Other than transport, the mRNP complex plays a major role in the generation,
maintenance, and regulation of the axonal transcriptome. Identification of axonal
RNA-binding proteins (RBPs) and analyses of the dynamics of their mRNPs are of
high interest to the field. Here, we describe methods for the study of
interactions between RNA and proteins in axons. First, we describe a protocol for
identifying binding proteins for an RNA of interest by using RNA affinity
chromatography. Subsequently, we discuss immunoprecipitation (IP) methods
allowing the dissection of protein-RNA and protein-protein interactions in mRNPs
under various physiological conditions.
free
free
free
