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2016 ; 1476
(ä): 117-28
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Isolating and Purifying Clostridium difficile Spores
#MMPMID27507337
Edwards AN
; McBride SM
Methods Mol Biol
2016[]; 1476
(ä): 117-28
PMID27507337
show ga
The ability for the obligate anaerobe, Clostridium difficile to form a
metabolically dormant spore is critical for the survival of this organism outside
of the host. This spore form is resistant to a myriad of environmental stresses,
including heat, desiccation, and exposure to disinfectants and antimicrobials.
These intrinsic properties of spores allow C. difficile to survive long-term in
an oxygenated environment, to be easily transmitted from host-to-host, and to
persist within the host following antibiotic treatment. Because of the importance
of the spore form to the C. difficile life cycle and treatment and prevention of
C. difficile infection (CDI), the isolation and purification of spores are
necessary to study the mechanisms of sporulation and germination, investigate
spore properties and resistances, and for use in animal models of CDI. Here we
provide basic protocols, in vitro growth conditions, and additional
considerations for purifying C. difficile spores for a variety of downstream
applications.
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