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2015 ; 7
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Ion homeostasis in a salt-secreting halophytic grass
#MMPMID25990364
Sanadhya P
; Agarwal P
; Agarwal PK
AoB Plants
2015[May]; 7
(ä): ä PMID25990364
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Salinity adversely affects plant growth and development, and disturbs
intracellular ion homeostasis, resulting in cellular toxicity. Plants that
tolerate salinity, halophytes, do so by manifesting numerous physiological and
biochemical processes in coordination to alleviate cellular ionic imbalance. The
present study was undertaken to analyse the salt tolerance mechanism in Aeluropus
lagopoides (L.) trin. Ex Thw. (Poaceae) at both physiological and molecular
levels. Plants secreted salt from glands, which eventually produced pristine salt
crystals on leaves and leaf sheaths. The rate of salt secretion increased with
increasing salt concentration in the growth medium. Osmotic adjustment was mainly
achieved by inorganic osmolytes (Na(+)) and at 100 mM NaCl no change was observed
in organic osmolytes in comparison to control plants. At 300 mM NaCl and with 150
mM NaCl + 150 mM KCl, the concentration of proline, soluble sugars and amino
acids was significantly increased. Transcript profiling of transporter genes
revealed differential spatial and temporal expressions in both shoot and root
tissues in a manner synchronized towards maintaining ion homeostasis. In shoots,
AlHKT2;1 transcript up-regulation was observed at 12 and 24 h in all the
treatments, whereas in roots, maximum induction was observed at 48 h with K(+)
starvation. The HAK transcript was relatively abundant in shoot tissue with all
the treatments. The plasma membrane Na(+)/H(+) antiporter, SOS1, and tonoplast
Na(+)/H(+) antiporter, NHX1, were found to be significantly up-regulated in shoot
tissue. Our data demonstrate that AlHKT2;1, HAK, SOS1, NHX1 and V-ATPase genes
play a pivotal role in regulating the ion homeostasis in A. lagopoides.