Investigating transcription reinitiation through in vitro approaches
#MMPMID25764113
Dieci G
; Fermi B
; Bosio MC
Transcription
2014[]; 5
(1
): e27704
PMID25764113
show ga
By influencing the number of RNA molecules repeatedly synthesized from the same
gene, the control of transcription reinitiation has the potential to shape the
transcriptome. Transcription reinitiation mechanisms have been mainly addressed
in vitro, through approaches based on both crude and reconstituted systems. These
studies support the notion that transcription reinitiation and its regulation
rely on dedicated networks of molecular interactions within transcription
machineries. At the same time, comparison with in vivo transcription rates
suggests that additional mechanisms, factors and conditions must exist in the
nucleus, whose biochemical elucidation is a fascinating challenge for future in
vitro transcription studies.
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