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10.1063/1.4954196 http://scihub22266oqcxt.onion/10.1063/1.4954196 C4912563!4912563 !27375817     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=27375817 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27375817 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Biomicrofluidics 2016 ; 10 (3 ): 033108 Nephropedia Template TP {{tp|p=27375817 |t=2016. Insulator-based dielectrophoretic diagnostic tool for babesiosis |pdf=|usr=}}{{27375817 }} gab.com Text Insulator-based dielectrophoretic diagnostic tool for babesiosis JO: Biomicrofluidics http://www.kidney.de/pget.php?&tw=1&pmid=27375817 #FOAvip Babesia species are obligate intraerythrocytic tick-borne protozoan parasites that are the etiologic agents of babesiosis, a potentially life-threatening, malaria-like illness in humans and animals. Babesia-infected people have been known to suffer from complications including liver problems, severe hemolytic anemia, and kidney failure. As reported by the Food and Drug Administration, 38% of mortality cases observed in transfusion recipients were associated with transfusion transmitted diseases of which babesiosis is the chief culprit. As of now, no tests have been licensed yet for screening blood donors for babesiosis. Current diagnostic tools for babesiosis including enzyme-linked immunosorbent assay, fluorescence in situ hybridization, and polymerase chain reaction are expensive and burdened with multifarious shortcomings. In this research, a low-cost, high-specificity, quick, and easy-to-use insulator-based dielectrophoretic diagnostic tool is developed for characterizing and concentrating Babesia-infected cells in their homogenous mixture with healthy cell population. In this work, a mixture of Babesia-infected (varying parasitemia) and healthy red blood cells (RBCs or erythrocytes) was exposed to non-uniform electric fields in a fabricated microfluidic platform to manipulate and sort the Babesia-infected cells within a minute. At DC voltage configurations of 10?V and 0/6?V in the inlet and the two outlet channels, respectively, the diseased cells were seen to flow in a direction different from the healthy RBCs. Bright field and fluorescence microscopy were utilized to present qualitative differentiation of the healthy erythrocytes from the infected cells. The proposed micro device platform was able to enrich RBCs from 0.1% to ?70% parasitemia. This device, when finally developed into a point-of-care diagnostic chip, would enhance the detection of Babesia-infected erythrocytes and as well serve as a precursor to babesiosis vaccine development. Twit Text FOAVip Insulator-based dielectrophoretic diagnostic tool for babesiosis ????Biomicrofluidics http://www.kidney.de/pget.php?&tw=1&pmid=27375817 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27375817 #FOAvip English Wikipedia <ref>{{Cite pmid|27375817 }}</ref> Insulator-based dielectrophoretic diagnostic tool for babesiosis #MMPMID27375817 Adekanmbi EO ; Ueti MW ; Rinaldi B ; Suarez CE ; Srivastava SK Biomicrofluidics 2016[May]; 10 (3 ): 033108 PMID27375817 show ga Babesia species are obligate intraerythrocytic tick-borne protozoan parasites that are the etiologic agents of babesiosis, a potentially life-threatening, malaria-like illness in humans and animals. Babesia-infected people have been known to suffer from complications including liver problems, severe hemolytic anemia, and kidney failure. As reported by the Food and Drug Administration, 38% of mortality cases observed in transfusion recipients were associated with transfusion transmitted diseases of which babesiosis is the chief culprit. As of now, no tests have been licensed yet for screening blood donors for babesiosis. Current diagnostic tools for babesiosis including enzyme-linked immunosorbent assay, fluorescence in situ hybridization, and polymerase chain reaction are expensive and burdened with multifarious shortcomings. In this research, a low-cost, high-specificity, quick, and easy-to-use insulator-based dielectrophoretic diagnostic tool is developed for characterizing and concentrating Babesia-infected cells in their homogenous mixture with healthy cell population. In this work, a mixture of Babesia-infected (varying parasitemia) and healthy red blood cells (RBCs or erythrocytes) was exposed to non-uniform electric fields in a fabricated microfluidic platform to manipulate and sort the Babesia-infected cells within a minute. At DC voltage configurations of 10?V and 0/6?V in the inlet and the two outlet channels, respectively, the diseased cells were seen to flow in a direction different from the healthy RBCs. Bright field and fluorescence microscopy were utilized to present qualitative differentiation of the healthy erythrocytes from the infected cells. The proposed micro device platform was able to enrich RBCs from 0.1% to ?70% parasitemia. This device, when finally developed into a point-of-care diagnostic chip, would enhance the detection of Babesia-infected erythrocytes and as well serve as a precursor to babesiosis vaccine development. äInsulator-based dielectrophoretic diagnostic tool for babesiosis (epmc).pdf DeepDyve Pubget Overpricing