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10.1038/srep17599 http://scihub22266oqcxt.onion/10.1038/srep17599 C4663473!4663473 !26616401     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26616401 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Sci+Rep 2015 ; 5 (ä): 17599 Nephropedia Template TP {{tp|p=26616401 |t=2015. Inhibition of insulin fibrillation by osmolytes: Mechanistic insights |pdf=|usr=}}{{26616401 }} gab.com Text Inhibition of insulin fibrillation by osmolytes: Mechanistic insights JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26616401 #FOAvip We have studied here using a number of biophysical tools the effects of osmolytes, betaine, citrulline, proline and sorbitol which differ significantly in terms of their physical characteristics such as, charge distribution, polarity, H-bonding abilities etc, on the fibrillation of insulin. Among these, betaine, citrulline, and proline are very effective in decreasing the extent of fibrillation. Proline also causes a substantial delay in the onset of fibrillation in the concentration range (50-250?mM) whereas such an effect is seen for citrulline only at 250?mM, and in case of betaine this effect is not seen at all in the whole concentration range. The enthalpies of interaction at various stages of fibrillation process have suggested that the preferential exclusion of the osmolyte and its polar interaction with the protein are important in inhibition. The results indicate that the osmolytes are most effective when added prior to the elongation stage of fibrillation. These observations have significant biological implications, since insulin fibrillation is known to cause injection amyloidosis and our data may help in designing lead drug molecules and development of potential therapeutic strategies. Twit Text FOAVip Inhibition of insulin fibrillation by osmolytes: Mechanistic insights ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26616401 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26616401 #FOAvip English Wikipedia <ref>{{Cite pmid|26616401 }}</ref> Inhibition of insulin fibrillation by osmolytes: Mechanistic insights #MMPMID26616401 Choudhary S ; Kishore N ; Hosur RV Sci Rep 2015[Nov]; 5 (ä): 17599 PMID26616401 show ga We have studied here using a number of biophysical tools the effects of osmolytes, betaine, citrulline, proline and sorbitol which differ significantly in terms of their physical characteristics such as, charge distribution, polarity, H-bonding abilities etc, on the fibrillation of insulin. Among these, betaine, citrulline, and proline are very effective in decreasing the extent of fibrillation. Proline also causes a substantial delay in the onset of fibrillation in the concentration range (50-250?mM) whereas such an effect is seen for citrulline only at 250?mM, and in case of betaine this effect is not seen at all in the whole concentration range. The enthalpies of interaction at various stages of fibrillation process have suggested that the preferential exclusion of the osmolyte and its polar interaction with the protein are important in inhibition. The results indicate that the osmolytes are most effective when added prior to the elongation stage of fibrillation. These observations have significant biological implications, since insulin fibrillation is known to cause injection amyloidosis and our data may help in designing lead drug molecules and development of potential therapeutic strategies. |*Osmolar Concentration [MESH] |Amyloid/*metabolism/ultrastructure [MESH] |Betaine/chemistry/metabolism [MESH] |Calorimetry [MESH] |Insulin/chemistry/*metabolism [MESH] |Kinetics [MESH] |Models, Biological [MESH] |Proline/chemistry/metabolism [MESH] |Protein Aggregation, Pathological/*metabolism [MESH] |Protein Binding [MESH]Inhibition of insulin fibrillation by osmolytes: Mechanistic insights (epmc).pdf DeepDyve Pubget Overpricing