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10.1038/nprot.2014.180 http://scihub22266oqcxt.onion/10.1038/nprot.2014.180 C5619698!5619698 !25321410     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=25321410 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25321410 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nat+Protoc 2014 ; 9 (11 ): 2663-81 Nephropedia Template TP {{tp|p=25321410 |t=2014. Influenza A virus isolation, culture and identification |pdf=|usr=}}{{25321410 }} gab.com Text Influenza A virus isolation, culture and identification JO: Nat Protoc http://www.kidney.de/pget.php?&tw=1&pmid=25321410 #FOAvip Influenza A viruses (IAVs) cause epidemics and pandemics that result in considerable financial burden and loss of human life. To manage annual IAV epidemics and prepare for future pandemics, an improved understanding of how IAVs emerge, transmit, cause disease and acquire pandemic potential is urgently needed. Fundamental techniques essential for procuring such knowledge are IAV isolation and culture from experimental and surveillance samples. Here we present a detailed protocol for IAV sample collection and processing, amplification in chicken eggs or mammalian cells, and identification from samples containing unknown pathogens. This protocol is robust, and it allows for the generation of virus cultures that can be used for downstream analyses. Once experimental or surveillance samples are obtained, virus cultures can be generated and the presence of IAVs can be verified in 3-5 d via reverse-transcription (RT)-PCR or hemagglutination assay. Increased time frames may be required for less experienced laboratory personnel, or when large numbers of samples will be processed. Twit Text FOAVip Influenza A virus isolation, culture and identification ????Nat Protoc http://www.kidney.de/pget.php?&tw=1&pmid=25321410 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25321410 #FOAvip English Wikipedia <ref>{{Cite pmid|25321410 }}</ref> Influenza A virus isolation, culture and identification #MMPMID25321410 Eisfeld AJ ; Neumann G ; Kawaoka Y Nat Protoc 2014[Nov]; 9 (11 ): 2663-81 PMID25321410 show ga Influenza A viruses (IAVs) cause epidemics and pandemics that result in considerable financial burden and loss of human life. To manage annual IAV epidemics and prepare for future pandemics, an improved understanding of how IAVs emerge, transmit, cause disease and acquire pandemic potential is urgently needed. Fundamental techniques essential for procuring such knowledge are IAV isolation and culture from experimental and surveillance samples. Here we present a detailed protocol for IAV sample collection and processing, amplification in chicken eggs or mammalian cells, and identification from samples containing unknown pathogens. This protocol is robust, and it allows for the generation of virus cultures that can be used for downstream analyses. Once experimental or surveillance samples are obtained, virus cultures can be generated and the presence of IAVs can be verified in 3-5 d via reverse-transcription (RT)-PCR or hemagglutination assay. Increased time frames may be required for less experienced laboratory personnel, or when large numbers of samples will be processed. |Animals [MESH] |Chick Embryo/virology [MESH] |Dogs [MESH] |Hemagglutination Tests [MESH] |Hemagglutination, Viral [MESH] |Influenza A virus/*genetics/*isolation & purification [MESH] |Madin Darby Canine Kidney Cells/virology [MESH] |Mammals/virology [MESH] |Reverse Transcriptase Polymerase Chain Reaction/methods [MESH]Influenza A virus isolation, culture and identification (epmc).pdf DeepDyve Pubget Overpricing