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2017 ; 4
(Pt 2
): 108-118
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In-cell NMR: a topical review
#MMPMID28250949
Luchinat E
; Banci L
IUCrJ
2017[Mar]; 4
(Pt 2
): 108-118
PMID28250949
show ga
Classical structural biology approaches allow structural characterization of
biological macromolecules in vitro, far from their physiological context.
Nowadays, thanks to the wealth of structural data available and to technological
and methodological advances, the interest of the research community is gradually
shifting from pure structural determination towards the study of functional
aspects of biomolecules. Therefore, a cellular structural approach is ideally
needed to characterize biological molecules, such as proteins, in their native
cellular environment and the functional processes that they are involved in.
In-cell NMR is a new application of high-resolution nuclear magnetic resonance
spectroscopy that allows structural and dynamical features of proteins and other
macromolecules to be analyzed directly in living cells. Owing to its challenging
nature, this methodology has shown slow, but steady, development over the past 15
years. To date, several in-cell NMR approaches have been successfully applied to
both bacterial and eukaryotic cells, including several human cell lines, and
important structural and functional aspects have been elucidated. In this topical
review, the major advances of in-cell NMR are summarized, with a special focus on
recent developments in eukaryotic and mammalian cells.
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