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10.1038/nature14299 http://scihub22266oqcxt.onion/10.1038/nature14299 C4393360!4393360 !25830891     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=25830891 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25830891 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nature 2015 ; 520 (7546 ): 186-91 Nephropedia Template TP {{tp|p=25830891 |t=2015. In vivo genome editing using Staphylococcus aureus Cas9 |pdf=|usr=}}{{25830891 }} gab.com Text In vivo genome editing using Staphylococcus aureus Cas9 JO: Nature http://www.kidney.de/pget.php?&tw=1&pmid=25830891 #FOAvip The RNA-guided endonuclease Cas9 has emerged as a versatile genome-editing platform. However, the size of the commonly used Cas9 from Streptococcus pyogenes (SpCas9) limits its utility for basic research and therapeutic applications that use the highly versatile adeno-associated virus (AAV) delivery vehicle. Here, we characterize six smaller Cas9 orthologues and show that Cas9 from Staphylococcus aureus (SaCas9) can edit the genome with efficiencies similar to those of SpCas9, while being more than 1 kilobase shorter. We packaged SaCas9 and its single guide RNA expression cassette into a single AAV vector and targeted the cholesterol regulatory gene Pcsk9 in the mouse liver. Within one week of injection, we observed >40% gene modification, accompanied by significant reductions in serum Pcsk9 and total cholesterol levels. We further assess the genome-wide targeting specificity of SaCas9 and SpCas9 using BLESS, and demonstrate that SaCas9-mediated in vivo genome editing has the potential to be efficient and specific. Twit Text FOAVip In vivo genome editing using Staphylococcus aureus Cas9 ????Nature http://www.kidney.de/pget.php?&tw=1&pmid=25830891 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25830891 #FOAvip English Wikipedia <ref>{{Cite pmid|25830891 }}</ref> In vivo genome editing using Staphylococcus aureus Cas9 #MMPMID25830891 Ran FA ; Cong L ; Yan WX ; Scott DA ; Gootenberg JS ; Kriz AJ ; Zetsche B ; Shalem O ; Wu X ; Makarova KS ; Koonin EV ; Sharp PA ; Zhang F Nature 2015[Apr]; 520 (7546 ): 186-91 PMID25830891 show ga The RNA-guided endonuclease Cas9 has emerged as a versatile genome-editing platform. However, the size of the commonly used Cas9 from Streptococcus pyogenes (SpCas9) limits its utility for basic research and therapeutic applications that use the highly versatile adeno-associated virus (AAV) delivery vehicle. Here, we characterize six smaller Cas9 orthologues and show that Cas9 from Staphylococcus aureus (SaCas9) can edit the genome with efficiencies similar to those of SpCas9, while being more than 1 kilobase shorter. We packaged SaCas9 and its single guide RNA expression cassette into a single AAV vector and targeted the cholesterol regulatory gene Pcsk9 in the mouse liver. Within one week of injection, we observed >40% gene modification, accompanied by significant reductions in serum Pcsk9 and total cholesterol levels. We further assess the genome-wide targeting specificity of SaCas9 and SpCas9 using BLESS, and demonstrate that SaCas9-mediated in vivo genome editing has the potential to be efficient and specific. |Animals [MESH] |Base Sequence [MESH] |CRISPR-Associated Proteins/genetics/*metabolism [MESH] |Cholesterol/blood/metabolism [MESH] |Gene Targeting [MESH] |Genetic Engineering/*methods [MESH] |Genome/*genetics [MESH] |Liver/metabolism/physiology [MESH] |Male [MESH] |Mice [MESH] |Mice, Inbred C57BL [MESH] |Proprotein Convertase 9 [MESH] |Proprotein Convertases/biosynthesis/blood/deficiency/genetics [MESH] |Serine Endopeptidases/biosynthesis/blood/deficiency/genetics [MESH] |Staphylococcus aureus/*enzymology/genetics [MESH]In vivo genome editing using Staphylococcus aureus Cas9 (epmc).pdf DeepDyve Pubget Overpricing