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10.1007/978-1-61779-163-5_4 http://scihub22266oqcxt.onion/10.1007/978-1-61779-163-5_4 C4794997!4794997 !21761293     free     free     free Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\21761293 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Methods+Mol+Biol 2011 ; 755 (ä): 57-66 Nephropedia Template TP {{tp|p=21761293 |t=2011. Immunoguided microdissection techniques |pdf=|usr=}}{{21761293 }} gab.com Text Immunoguided microdissection techniques JO: Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=21761293 #FOAvip Over the past 15 years, laser-based microdissection has improved the precision by which scientists can procure cells of interest from a heterogeneous tissue section. However, for studies that require a large amount of material (e.g., proteomics) or for cells that are scattered and difficult to identify by standard histological stains, an immunostain-based, automated approach becomes essential. In this chapter, we discuss the use of immunohistochemistry (IHC) and immunofluorescence (IF) to guide the microdissection process via manual and software-driven auto-dissection methods. Although technical challenges still exist with these innovative approaches, we present here methods and protocols to successfully perform immuno-based microdissection on commercially available laser dissection systems. Twit Text FOAVip Immunoguided microdissection techniques ????Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=21761293 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=21761293 #FOAvip English Wikipedia <ref>{{Cite pmid|21761293 }}</ref> Immunoguided microdissection techniques #MMPMID21761293 Tangrea MA ; Hanson JC ; Bonner RF ; Pohida TJ ; Rodriguez-Canales J ; Emmert-Buck MR Methods Mol Biol 2011[]; 755 (ä): 57-66 PMID21761293 show ga Over the past 15 years, laser-based microdissection has improved the precision by which scientists can procure cells of interest from a heterogeneous tissue section. However, for studies that require a large amount of material (e.g., proteomics) or for cells that are scattered and difficult to identify by standard histological stains, an immunostain-based, automated approach becomes essential. In this chapter, we discuss the use of immunohistochemistry (IHC) and immunofluorescence (IF) to guide the microdissection process via manual and software-driven auto-dissection methods. Although technical challenges still exist with these innovative approaches, we present here methods and protocols to successfully perform immuno-based microdissection on commercially available laser dissection systems. |*Lasers [MESH] |Cell Separation/methods [MESH] |Cryopreservation [MESH] |Humans [MESH] |Image Processing, Computer-Assisted/methods [MESH] |Immunohistochemistry/*methods [MESH] |Keratins/metabolism [MESH] |Male [MESH] |Microdissection/*methods [MESH] |Paraffin Embedding [MESH] |Prostate/cytology/metabolism [MESH] |Software [MESH]Immunoguided microdissection techniques (epmc).pdf DeepDyve Pubget Overpricing