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10.1016/B978-0-12-388448-0.00029-2 http://scihub22266oqcxt.onion/10.1016/B978-0-12-388448-0.00029-2 C5014494!5014494 !22289465     free     free     free Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\22289465 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Methods+Enzymol 2012 ; 505 (ä): 401-19 Nephropedia Template TP {{tp|p=22289465 |t=2012. Imaging the glycome in living systems |pdf=|usr=}}{{22289465 }} gab.com Text Imaging the glycome in living systems JO: Methods Enzymol http://www.kidney.de/pget.php?&tw=1&pmid=22289465 #FOAvip The glycome, the full complement of glycans that cells produce, is an attractive target for molecular imaging. Imaging of the glycome in living systems has recently been enabled via bioorthogonal chemical reporter-based approaches. In this chapter, we describe two approaches to introduce bioorthogonal chemical reporters (tags) onto cell surface fucosylated glycans and glycans bearing LacNAc disaccharides, respectively. The tagged glycans can then be conjugated to imaging probes via bioorthogonal click chemistry. Similar approaches can be extended to image other sectors of the glycome in living systems. Twit Text FOAVip Imaging the glycome in living systems ????Methods Enzymol http://www.kidney.de/pget.php?&tw=1&pmid=22289465 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=22289465 #FOAvip English Wikipedia <ref>{{Cite pmid|22289465 }}</ref> Imaging the glycome in living systems #MMPMID22289465 Li B ; Mock F ; Wu P Methods Enzymol 2012[]; 505 (ä): 401-19 PMID22289465 show ga The glycome, the full complement of glycans that cells produce, is an attractive target for molecular imaging. Imaging of the glycome in living systems has recently been enabled via bioorthogonal chemical reporter-based approaches. In this chapter, we describe two approaches to introduce bioorthogonal chemical reporters (tags) onto cell surface fucosylated glycans and glycans bearing LacNAc disaccharides, respectively. The tagged glycans can then be conjugated to imaging probes via bioorthogonal click chemistry. Similar approaches can be extended to image other sectors of the glycome in living systems. |Animals [MESH] |Azides/chemistry [MESH] |Cell Membrane/chemistry/metabolism [MESH] |Cell Tracking/*methods [MESH] |Click Chemistry/*methods [MESH] |Fluorescent Dyes [MESH] |Microscopy, Confocal/*methods [MESH] |Polysaccharides/*analysis/chemistry [MESH] |Staining and Labeling/methods [MESH]Imaging the glycome in living systems (epmc).pdf DeepDyve Pubget Overpricing