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Imaging methodologies for systems biology
#MMPMID25482526
Smith SE
; Slaughter BD
; Unruh JR
Cell Adh Migr
2014[]; 8
(5
): 468-77
PMID25482526
show ga
Systems biology has recently achieved significant success in the understanding of
complex interconnected phenomena such as cell polarity and migration. In this
context, the definition of systems biology has come to encompass the integration
of quantitative measurements with sophisticated modeling approaches. This article
will review recent progress in live cell imaging technologies that have expanded
the possibilities of quantitative in vivo measurements, particularly in regards
to molecule counting and quantitative measurements of protein concentration and
dynamics. These methods have gained and continue to gain popularity with the
biological community. In general, we will discuss three broad categories: protein
interactions, protein quantitation, and protein dynamics.
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