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Imaging mRNA and protein interactions within neurons #MMPMID28223507
Eliscovich C; Shenoy SM; Singer RH
Proc Natl Acad Sci U S A 2017[Mar]; 114 (10): E1875-84 PMID28223507show ga
Gene expression is regulated by interactions between mRNAs and RNA-binding proteins. This functionality depends on their ability to specifically recognize and bind RNAs within the cell. Thus, elucidation of RNA?protein interactions is an area of active research. Technological developments allowed the study of these associations in living cells by ensemble biochemistry approaches. However, these traditional methods to investigate RNA?protein association may report adventitious associations and importantly, lack information about cellular spatial information. In this work, we provide an approach that can integrate information from biochemical interactions with visualization of these physical contacts within neurons.