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2014 ; 5
(6
): 1822-38
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Image simulation for biological microscopy: microlith
#MMPMID24940543
Mehta SB
; Oldenbourg R
Biomed Opt Express
2014[Jun]; 5
(6
): 1822-38
PMID24940543
show ga
Image simulation remains under-exploited for the most widely used biological
phase microscopy methods, because of difficulties in simulating partially
coherent illumination. We describe an open-source toolbox, microlith
(https://code.google.com/p/microlith), which accurately predicts
three-dimensional images of a thin specimen observed with any partially coherent
imaging system, as well as images of coherently illuminated and self-luminous
incoherent specimens. Its accuracy is demonstrated by comparing simulated and
experimental bright-field and dark-field images of well-characterized amplitude
and phase targets, respectively. The comparison provides new insights about the
sensitivity of the dark-field microscope to mass distributions in isolated or
periodic specimens at the length-scale of 10nm. Based on predictions using
microlith, we propose a novel approach for detecting nanoscale structural changes
in a beating axoneme using a dark-field microscope.
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