Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=28514427
&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215
Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28514427
.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Nat+Chem+Biol
2017 ; 13
(6
): 587-598
Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Illuminating developmental biology through photochemistry
#MMPMID28514427
Kowalik L
; Chen JK
Nat Chem Biol
2017[May]; 13
(6
): 587-598
PMID28514427
show ga
Developmental biology has been continually shaped by technological advances,
evolving from a descriptive science into one immersed in molecular and cellular
mechanisms. Most recently, genome sequencing and 'omics' profiling have provided
developmental biologists with a wealth of genetic and biochemical information;
however, fully translating this knowledge into functional understanding will
require new experimental capabilities. Photoactivatable probes have emerged as
particularly valuable tools for investigating developmental mechanisms, as they
can enable rapid, specific manipulations of DNA, RNA, proteins, and cells with
spatiotemporal precision. In this Perspective, we describe optochemical and
optogenetic systems that have been applied in multicellular organisms, insights
gained through the use of these probes, and their current limitations. We also
suggest how chemical biologists can expand the reach of photoactivatable
technologies and bring new depth to our understanding of organismal development.
free
free
free
