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10.1089/nat.2014.0522 http://scihub22266oqcxt.onion/10.1089/nat.2014.0522 C4440997!4440997 !25894736     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25894736 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nucleic+Acid+Ther 2015 ; 25 (3 ): 162-72 Nephropedia Template TP {{tp|p=25894736 |t=2015. Identification of epithelial ovarian tumor-specific aptamers |pdf=|usr=}}{{25894736 }} gab.com Text Identification of epithelial ovarian tumor-specific aptamers JO: Nucleic Acid Ther http://www.kidney.de/pget.php?&tw=1&pmid=25894736 #FOAvip Ovarian cancer is often diagnosed in late stages with few treatment options and poor long-term prognosis. New clinical tools for early detection of ovarian malignancies will significantly help reduce mortality and improve current long-term survival rates. The objective of this work was to identify ovarian tumor-specific single-stranded DNA aptamers that bind to malignant ovarian tumor cells and internalize with high affinity and specificity. Aptamers can identify unique tumor biomarkers, can aid in early detection and diagnosis of neoplastic disorders, and can be functionalized by conjugation to small molecules. To identify aptamers from random single-stranded DNA pools (60 bases long), we used whole Cell-SELEX (systematic evolution of ligands by exponential enrichment) to enrich and isolate tumor-specific aptamers that bind to tumor-specific receptors in their native state on the cell surface. Next-Generation sequencing identified seven novel aptamers and detailed analyses of three are described. Aptamers bound to, and were internalized by, target Caov-3 cell populations, but not nontarget nonmalignant ovarian epithelial HOSE 6-3 cells or multiple other epithelial tumor cell lines. Furthermore, aptamers showed unique binding affinities with apparent dissociation constants (Kd) measuring in the submicromolar range supporting their physiological relevance and potential use in clinical applications. Twit Text FOAVip Identification of epithelial ovarian tumor-specific aptamers ????Nucleic Acid Ther http://www.kidney.de/pget.php?&tw=1&pmid=25894736 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25894736 #FOAvip English Wikipedia <ref>{{Cite pmid|25894736 }}</ref> Identification of epithelial ovarian tumor-specific aptamers #MMPMID25894736 Benedetto G ; Hamp TJ ; Wesselman PJ ; Richardson C Nucleic Acid Ther 2015[Jun]; 25 (3 ): 162-72 PMID25894736 show ga Ovarian cancer is often diagnosed in late stages with few treatment options and poor long-term prognosis. New clinical tools for early detection of ovarian malignancies will significantly help reduce mortality and improve current long-term survival rates. The objective of this work was to identify ovarian tumor-specific single-stranded DNA aptamers that bind to malignant ovarian tumor cells and internalize with high affinity and specificity. Aptamers can identify unique tumor biomarkers, can aid in early detection and diagnosis of neoplastic disorders, and can be functionalized by conjugation to small molecules. To identify aptamers from random single-stranded DNA pools (60 bases long), we used whole Cell-SELEX (systematic evolution of ligands by exponential enrichment) to enrich and isolate tumor-specific aptamers that bind to tumor-specific receptors in their native state on the cell surface. Next-Generation sequencing identified seven novel aptamers and detailed analyses of three are described. Aptamers bound to, and were internalized by, target Caov-3 cell populations, but not nontarget nonmalignant ovarian epithelial HOSE 6-3 cells or multiple other epithelial tumor cell lines. Furthermore, aptamers showed unique binding affinities with apparent dissociation constants (Kd) measuring in the submicromolar range supporting their physiological relevance and potential use in clinical applications. |Aptamers, Nucleotide/*genetics [MESH] |Carcinoma, Ovarian Epithelial [MESH] |Cell Line, Tumor [MESH] |Female [MESH] |Humans [MESH] |Neoplasms, Glandular and Epithelial/*genetics/pathology [MESH] |Ovarian Neoplasms/*genetics/pathology [MESH]Identification of epithelial ovarian tumor-specific aptamers (epmc).pdf DeepDyve Pubget Overpricing