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10.1038/ncomms8990 http://scihub22266oqcxt.onion/10.1038/ncomms8990 C4569691!4569691 !26329685     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26329685 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26329685 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nat+Commun 2015 ; 6 (ä): 7990 Nephropedia Template TP {{tp|p=26329685 |t=2015. Hyperspectral light sheet microscopy |pdf=|usr=}}{{26329685 }} gab.com Text Hyperspectral light sheet microscopy JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=26329685 #FOAvip To study the development and interactions of cells and tissues, multiple fluorescent markers need to be imaged efficiently in a single living organism. Instead of acquiring individual colours sequentially with filters, we created a platform based on line-scanning light sheet microscopy to record the entire spectrum for each pixel in a three-dimensional volume. We evaluated data sets with varying spectral sampling and determined the optimal channel width to be around 5?nm. With the help of these data sets, we show that our setup outperforms filter-based approaches with regard to image quality and discrimination of fluorophores. By spectral unmixing we resolved overlapping fluorophores with up to nanometre resolution and removed autofluorescence in zebrafish and fruit fly embryos. Twit Text FOAVip Hyperspectral light sheet microscopy ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=26329685 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26329685 #FOAvip English Wikipedia <ref>{{Cite pmid|26329685 }}</ref> Hyperspectral light sheet microscopy #MMPMID26329685 Jahr W ; Schmid B ; Schmied C ; Fahrbach FO ; Huisken J Nat Commun 2015[Sep]; 6 (ä): 7990 PMID26329685 show ga To study the development and interactions of cells and tissues, multiple fluorescent markers need to be imaged efficiently in a single living organism. Instead of acquiring individual colours sequentially with filters, we created a platform based on line-scanning light sheet microscopy to record the entire spectrum for each pixel in a three-dimensional volume. We evaluated data sets with varying spectral sampling and determined the optimal channel width to be around 5?nm. With the help of these data sets, we show that our setup outperforms filter-based approaches with regard to image quality and discrimination of fluorophores. By spectral unmixing we resolved overlapping fluorophores with up to nanometre resolution and removed autofluorescence in zebrafish and fruit fly embryos. |*Drosophila melanogaster [MESH] |*Embryo, Nonmammalian [MESH] |*Zebrafish [MESH] |Animals [MESH] |Fluorescent Dyes [MESH] |Image Processing, Computer-Assisted [MESH] |Microscopy, Fluorescence/*methods [MESH]Hyperspectral light sheet microscopy (epmc).pdf DeepDyve Pubget Overpricing