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Hydrogen sulfide accelerates wound healing in diabetic rats
#MMPMID26191204
Wang G
; Li W
; Chen Q
; Jiang Y
; Lu X
; Zhao X
Int J Clin Exp Pathol
2015[]; 8
(5
): 5097-104
PMID26191204
show ga
AIM: The aim of this study was to explore the role of hydrogen sulfide on wound
healing in diabetic rats. METHODS: Experimental diabetes in rats was induced by
intraperitoneal injection of streptozotocin (STZ) (in 0.1 mol/L citrate buffer,
Ph 4.5) at dose of 70 mg/kg. Diabetic and age-matched non-diabetic rats were
randomly assigned to three groups: untreated diabetic controls (UDC), treated
diabetic administrations (TDA), and non-diabetic controls (NDC). Wound Healing
Model was prepared by making a round incision (2.0 cm in diameter) in full
thickness. Rats from TDA receive 2% sodium bisulfide ointment on wound, and
animals from UDC and NDC receive control cream. After treatment of 21 days with
sodium bisulfide, blood samples were collected for determination of vascular
endothelial growth factor (VEGF), intercellular cell adhesion molecule-1
(ICAM-1), antioxidant effects. Granulation tissues from the wound were processed
for histological examination and analysis of western blot. RESULTS: The study
indicated a significant increase in levels of VEGF and ICAM-1 and a decline in
activity of coagulation in diabetic rats treated with sodium bisulfide. Sodium
bisulfide treatment raised the activity of superoxide dismutase (SOD) and heme
oxygenase-1 (HO-1) protein expression, and decreased tumor necrosis factor ?
(TNF-?) protein expression in diabetic rats. CONCLUSIONS: The findings in present
study suggested that hydrogen sulfide accelerates the wound healing in rats with
diabetes. The beneficial effect of H2S may be associated with formation of
granulation, anti-inflammation, antioxidant, and the increased level of vascular
endothelial growth factor (VEGF).
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