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10.1038/s41467-018-05067-x http://scihub22266oqcxt.onion/10.1038/s41467-018-05067-x C6035213!6035213 !29980666     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\29980666 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nat+Commun 2018 ; 9 (1 ): 2643 Nephropedia Template TP {{tp|p=29980666 |t=2018. Human pluripotent reprogramming with CRISPR activators |pdf=|usr=}}{{29980666 }} gab.com Text Human pluripotent reprogramming with CRISPR activators JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=29980666 #FOAvip CRISPR-Cas9-based gene activation (CRISPRa) is an attractive tool for cellular reprogramming applications due to its high multiplexing capacity and direct targeting of endogenous loci. Here we present the reprogramming of primary human skin fibroblasts into induced pluripotent stem cells (iPSCs) using CRISPRa, targeting endogenous OCT4, SOX2, KLF4, MYC, and LIN28A promoters. The low basal reprogramming efficiency can be improved by an order of magnitude by additionally targeting a conserved Alu-motif enriched near genes involved in embryo genome activation (EEA-motif). This effect is mediated in part by more efficient activation of NANOG and REX1. These data demonstrate that human somatic cells can be reprogrammed into iPSCs using only CRISPRa. Furthermore, the results unravel the involvement of EEA-motif-associated mechanisms in cellular reprogramming. Twit Text FOAVip Human pluripotent reprogramming with CRISPR activators ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=29980666 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29980666 #FOAvip English Wikipedia <ref>{{Cite pmid|29980666 }}</ref> Human pluripotent reprogramming with CRISPR activators #MMPMID29980666 Weltner J ; Balboa D ; Katayama S ; Bespalov M ; Krjut?kov K ; Jouhilahti EM ; Trokovic R ; Kere J ; Otonkoski T Nat Commun 2018[Jul]; 9 (1 ): 2643 PMID29980666 show ga CRISPR-Cas9-based gene activation (CRISPRa) is an attractive tool for cellular reprogramming applications due to its high multiplexing capacity and direct targeting of endogenous loci. Here we present the reprogramming of primary human skin fibroblasts into induced pluripotent stem cells (iPSCs) using CRISPRa, targeting endogenous OCT4, SOX2, KLF4, MYC, and LIN28A promoters. The low basal reprogramming efficiency can be improved by an order of magnitude by additionally targeting a conserved Alu-motif enriched near genes involved in embryo genome activation (EEA-motif). This effect is mediated in part by more efficient activation of NANOG and REX1. These data demonstrate that human somatic cells can be reprogrammed into iPSCs using only CRISPRa. Furthermore, the results unravel the involvement of EEA-motif-associated mechanisms in cellular reprogramming. |Alu Elements/genetics [MESH] |Base Sequence [MESH] |CRISPR-Associated Protein 9/*metabolism [MESH] |Cellular Reprogramming/*genetics [MESH] |Clustered Regularly Interspaced Short Palindromic Repeats/*genetics [MESH] |Embryo, Mammalian/metabolism [MESH] |Fibroblasts/metabolism [MESH] |Gene Expression Regulation, Developmental [MESH] |HEK293 Cells [MESH] |Humans [MESH] |Induced Pluripotent Stem Cells/*cytology/*metabolism [MESH] |Kruppel-Like Factor 4 [MESH] |Male [MESH] |Nanog Homeobox Protein/metabolism [MESH] |Neural Stem Cells/metabolism [MESH] |Nucleotide Motifs/genetics [MESH] |RNA, Guide, CRISPR-Cas Systems/metabolism [MESH]Human pluripotent reprogramming with CRISPR activators (epmc).pdf DeepDyve Pubget Overpricing