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10.1091/mbc.E18-01-0016 http://scihub22266oqcxt.onion/10.1091/mbc.E18-01-0016 C5935067!5935067 !29540526     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\29540526 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Mol+Biol+Cell 2018 ; 29 (10 ): 1168-1177 Nephropedia Template TP {{tp|p=29540526 |t=2018. Human CLASP2 specifically regulates microtubule catastrophe and rescue |pdf=|usr=}}{{29540526 }} gab.com Text Human CLASP2 specifically regulates microtubule catastrophe and rescue JO: Mol Biol Cell http://www.kidney.de/pget.php?&tw=1&pmid=29540526 #FOAvip Cytoplasmic linker-associated proteins (CLASPs) are microtubule-associated proteins essential for microtubule regulation in many cellular processes. However, the molecular mechanisms underlying CLASP activity are not understood. Here, we use purified protein components and total internal reflection fluorescence microscopy to investigate the effects of human CLASP2 on microtubule dynamics in vitro. We demonstrate that CLASP2 suppresses microtubule catastrophe and promotes rescue without affecting the rates of microtubule growth or shrinkage. Strikingly, when CLASP2 is combined with EB1, a known binding partner, the effects on microtubule dynamics are strongly enhanced. We show that synergy between CLASP2 and EB1 is dependent on a direct interaction, since a truncated EB1 protein that lacks the CLASP2-binding domain does not enhance CLASP2 activity. Further, we find that EB1 targets CLASP2 to microtubules and increases the dwell time of CLASP2 at microtubule tips. Although the temporally averaged microtubule growth rates are unaffected by CLASP2, we find that microtubules grown with CLASP2 display greater variability in growth rates. Our results provide insight into the regulation of microtubule dynamics by CLASP proteins and highlight the importance of the functional interplay between regulatory proteins at dynamic microtubule ends. Twit Text FOAVip Human CLASP2 specifically regulates microtubule catastrophe and rescue ????Mol Biol Cell http://www.kidney.de/pget.php?&tw=1&pmid=29540526 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=29540526 #FOAvip English Wikipedia <ref>{{Cite pmid|29540526 }}</ref> Human CLASP2 specifically regulates microtubule catastrophe and rescue #MMPMID29540526 Lawrence EJ ; Arpag G ; Norris SR ; Zanic M Mol Biol Cell 2018[May]; 29 (10 ): 1168-1177 PMID29540526 show ga Cytoplasmic linker-associated proteins (CLASPs) are microtubule-associated proteins essential for microtubule regulation in many cellular processes. However, the molecular mechanisms underlying CLASP activity are not understood. Here, we use purified protein components and total internal reflection fluorescence microscopy to investigate the effects of human CLASP2 on microtubule dynamics in vitro. We demonstrate that CLASP2 suppresses microtubule catastrophe and promotes rescue without affecting the rates of microtubule growth or shrinkage. Strikingly, when CLASP2 is combined with EB1, a known binding partner, the effects on microtubule dynamics are strongly enhanced. We show that synergy between CLASP2 and EB1 is dependent on a direct interaction, since a truncated EB1 protein that lacks the CLASP2-binding domain does not enhance CLASP2 activity. Further, we find that EB1 targets CLASP2 to microtubules and increases the dwell time of CLASP2 at microtubule tips. Although the temporally averaged microtubule growth rates are unaffected by CLASP2, we find that microtubules grown with CLASP2 display greater variability in growth rates. Our results provide insight into the regulation of microtubule dynamics by CLASP proteins and highlight the importance of the functional interplay between regulatory proteins at dynamic microtubule ends. |Animals [MESH] |Cattle [MESH] |Humans [MESH] |Microtubule-Associated Proteins/*metabolism [MESH] |Microtubules/*metabolism [MESH] |Polymerization [MESH] |Protein Binding [MESH] |Solubility [MESH]Human CLASP2 specifically regulates microtubule catastrophe and rescue(epmc).pdf DeepDyve Pubget Overpricing