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10.1016/j.chembiol.2015.09.015 http://scihub22266oqcxt.onion/10.1016/j.chembiol.2015.09.015 C4756654!4756654 !26548613     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26548613 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26548613 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Chem+Biol 2015 ; 22 (11 ): 1521-1530 Nephropedia Template TP {{tp|p=26548613 |t=2015. Host-Microbe Protein Interactions during Bacterial Infection |pdf=|usr=}}{{26548613 }} gab.com Text Host-Microbe Protein Interactions during Bacterial Infection JO: Chem Biol http://www.kidney.de/pget.php?&tw=1&pmid=26548613 #FOAvip Interspecies protein-protein interactions are essential mediators of infection. While bacterial proteins required for host cell invasion and infection can be identified through bacterial mutant library screens, information about host target proteins and interspecies complex structures has been more difficult to acquire. Using an unbiased chemical crosslinking/mass spectrometry approach, we identified interspecies protein-protein interactions in human lung epithelial cells infected with Acinetobacter baumannii. These efforts resulted in identification of 3,076 crosslinked peptide pairs and 46 interspecies protein-protein interactions. Most notably, the key A. baumannii virulence factor, OmpA, was identified as crosslinked to host proteins involved in desmosomes, specialized structures that mediate host cell-to-cell adhesion. Co-immunoprecipitation and transposon mutant experiments were used to verify these interactions and demonstrate relevance for host cell invasion and acute murine lung infection. These results shed new light on A. baumannii-host protein interactions and their structural features, and the presented approach is generally applicable to other systems. Twit Text FOAVip Host-Microbe Protein Interactions during Bacterial Infection ????Chem Biol http://www.kidney.de/pget.php?&tw=1&pmid=26548613 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26548613 #FOAvip English Wikipedia <ref>{{Cite pmid|26548613 }}</ref> Host-Microbe Protein Interactions during Bacterial Infection #MMPMID26548613 Schweppe DK ; Harding C ; Chavez JD ; Wu X ; Ramage E ; Singh PK ; Manoil C ; Bruce JE Chem Biol 2015[Nov]; 22 (11 ): 1521-1530 PMID26548613 show ga Interspecies protein-protein interactions are essential mediators of infection. While bacterial proteins required for host cell invasion and infection can be identified through bacterial mutant library screens, information about host target proteins and interspecies complex structures has been more difficult to acquire. Using an unbiased chemical crosslinking/mass spectrometry approach, we identified interspecies protein-protein interactions in human lung epithelial cells infected with Acinetobacter baumannii. These efforts resulted in identification of 3,076 crosslinked peptide pairs and 46 interspecies protein-protein interactions. Most notably, the key A. baumannii virulence factor, OmpA, was identified as crosslinked to host proteins involved in desmosomes, specialized structures that mediate host cell-to-cell adhesion. Co-immunoprecipitation and transposon mutant experiments were used to verify these interactions and demonstrate relevance for host cell invasion and acute murine lung infection. These results shed new light on A. baumannii-host protein interactions and their structural features, and the presented approach is generally applicable to other systems. |*Host-Pathogen Interactions [MESH] |Acinetobacter Infections/*microbiology/*physiopathology [MESH] |Acinetobacter baumannii/*physiology [MESH] |Animals [MESH] |Bacterial Outer Membrane Proteins/chemistry/metabolism [MESH] |Humans [MESH] |Mice [MESH] |Microscopy, Confocal [MESH] |Protein Interaction Domains and Motifs [MESH] |Protein Structure, Tertiary [MESH]Host-Microbe Protein Interactions during Bacterial Infection (epmc).pdf DeepDyve Pubget Overpricing