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10.1091/mbc.E14-07-1197 http://scihub22266oqcxt.onion/10.1091/mbc.E14-07-1197 C4436775!4436775 !25739453     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=25739453 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25739453 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Mol+Biol+Cell 2015 ; 26 (9 ): 1629-39 Nephropedia Template TP {{tp|p=25739453 |t=2015. Homer3 regulates the establishment of neutrophil polarity |pdf=|usr=}}{{25739453 }} gab.com Text Homer3 regulates the establishment of neutrophil polarity JO: Mol Biol Cell http://www.kidney.de/pget.php?&tw=1&pmid=25739453 #FOAvip Most chemoattractants rely on activation of the heterotrimeric G-protein G?i to regulate directional cell migration, but few links from G?i to chemotactic effectors are known. Through affinity chromatography using primary neutrophil lysate, we identify Homer3 as a novel G?i2-binding protein. RNA interference-mediated knockdown of Homer3 in neutrophil-like HL-60 cells impairs chemotaxis and the establishment of polarity of phosphatidylinositol 3,4,5-triphosphate (PIP3) and the actin cytoskeleton, as well as the persistence of the WAVE2 complex. Most previously characterized proteins that are required for cell polarity are needed for actin assembly or activation of core chemotactic effectors such as the Rac GTPase. In contrast, Homer3-knockdown cells show normal magnitude and kinetics of chemoattractant-induced activation of phosphoinositide 3-kinase and Rac effectors. Chemoattractant-stimulated Homer3-knockdown cells also exhibit a normal initial magnitude of actin polymerization but fail to polarize actin assembly and intracellular PIP3 and are defective in the initiation of cell polarity and motility. Our data suggest that Homer3 acts as a scaffold that spatially organizes actin assembly to support neutrophil polarity and motility downstream of GPCR activation. Twit Text FOAVip Homer3 regulates the establishment of neutrophil polarity ????Mol Biol Cell http://www.kidney.de/pget.php?&tw=1&pmid=25739453 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25739453 #FOAvip English Wikipedia <ref>{{Cite pmid|25739453 }}</ref> Homer3 regulates the establishment of neutrophil polarity #MMPMID25739453 Wu J ; Pipathsouk A ; Keizer-Gunnink A ; Fusetti F ; Alkema W ; Liu S ; Altschuler S ; Wu L ; Kortholt A ; Weiner OD Mol Biol Cell 2015[May]; 26 (9 ): 1629-39 PMID25739453 show ga Most chemoattractants rely on activation of the heterotrimeric G-protein G?i to regulate directional cell migration, but few links from G?i to chemotactic effectors are known. Through affinity chromatography using primary neutrophil lysate, we identify Homer3 as a novel G?i2-binding protein. RNA interference-mediated knockdown of Homer3 in neutrophil-like HL-60 cells impairs chemotaxis and the establishment of polarity of phosphatidylinositol 3,4,5-triphosphate (PIP3) and the actin cytoskeleton, as well as the persistence of the WAVE2 complex. Most previously characterized proteins that are required for cell polarity are needed for actin assembly or activation of core chemotactic effectors such as the Rac GTPase. In contrast, Homer3-knockdown cells show normal magnitude and kinetics of chemoattractant-induced activation of phosphoinositide 3-kinase and Rac effectors. Chemoattractant-stimulated Homer3-knockdown cells also exhibit a normal initial magnitude of actin polymerization but fail to polarize actin assembly and intracellular PIP3 and are defective in the initiation of cell polarity and motility. Our data suggest that Homer3 acts as a scaffold that spatially organizes actin assembly to support neutrophil polarity and motility downstream of GPCR activation. |Actin Cytoskeleton/metabolism [MESH] |Carrier Proteins/*physiology [MESH] |Cell Polarity [MESH] |Chemotaxis [MESH] |Gene Expression [MESH] |Gene Knockdown Techniques [MESH] |HL-60 Cells [MESH] |Homer Scaffolding Proteins [MESH] |Humans [MESH] |Neutrophils/*physiology [MESH] |RNA, Small Interfering/genetics [MESH]Homer3 regulates the establishment of neutrophil polarity (epmc).pdf DeepDyve Pubget Overpricing