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2018 ; 35
(5
): 361-368
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History of genome editing in yeast
#MMPMID29345746
Fraczek MG
; Naseeb S
; Delneri D
Yeast
2018[May]; 35
(5
): 361-368
PMID29345746
show ga
For thousands of years humans have used the budding yeast Saccharomyces
cerevisiae for the production of bread and alcohol; however, in the last
30-40 years our understanding of the yeast biology has dramatically increased,
enabling us to modify its genome. Although S. cerevisiae has been the main focus
of many research groups, other non-conventional yeasts have also been studied and
exploited for biotechnological purposes. Our experiments and knowledge have
evolved from recombination to high-throughput PCR-based transformations to highly
accurate CRISPR methods in order to alter yeast traits for either research or
industrial purposes. Since the release of the genome sequence of S. cerevisiae in
1996, the precise and targeted genome editing has increased significantly. In
this 'Budding topic' we discuss the significant developments of genome editing in
yeast, mainly focusing on Cre-loxP mediated recombination, delitto perfetto and
CRISPR/Cas.