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10.1038/nmeth.3312 http://scihub22266oqcxt.onion/10.1038/nmeth.3312 C4393883!4393883 !25730490     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25730490 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nat+Methods 2015 ; 12 (4 ): 326-8 Nephropedia Template TP {{tp|p=25730490 |t=2015. Highly efficient Cas9-mediated transcriptional programming |pdf=|usr=}}{{25730490 }} gab.com Text Highly efficient Cas9-mediated transcriptional programming JO: Nat Methods http://www.kidney.de/pget.php?&tw=1&pmid=25730490 #FOAvip The RNA-guided nuclease Cas9 can be reengineered as a programmable transcription factor. However, modest levels of gene activation have limited potential applications. We describe an improved transcriptional regulator obtained through the rational design of a tripartite activator, VP64-p65-Rta (VPR), fused to nuclease-null Cas9. We demonstrate its utility in activating endogenous coding and noncoding genes, targeting several genes simultaneously and stimulating neuronal differentiation of human induced pluripotent stem cells (iPSCs). Twit Text FOAVip Highly efficient Cas9-mediated transcriptional programming ????Nat Methods http://www.kidney.de/pget.php?&tw=1&pmid=25730490 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25730490 #FOAvip English Wikipedia <ref>{{Cite pmid|25730490 }}</ref> Highly efficient Cas9-mediated transcriptional programming #MMPMID25730490 Chavez A ; Scheiman J ; Vora S ; Pruitt BW ; Tuttle M ; P R Iyer E ; Lin S ; Kiani S ; Guzman CD ; Wiegand DJ ; Ter-Ovanesyan D ; Braff JL ; Davidsohn N ; Housden BE ; Perrimon N ; Weiss R ; Aach J ; Collins JJ ; Church GM Nat Methods 2015[Apr]; 12 (4 ): 326-8 PMID25730490 show ga The RNA-guided nuclease Cas9 can be reengineered as a programmable transcription factor. However, modest levels of gene activation have limited potential applications. We describe an improved transcriptional regulator obtained through the rational design of a tripartite activator, VP64-p65-Rta (VPR), fused to nuclease-null Cas9. We demonstrate its utility in activating endogenous coding and noncoding genes, targeting several genes simultaneously and stimulating neuronal differentiation of human induced pluripotent stem cells (iPSCs). |*Endonucleases/genetics [MESH] |*Genetic Techniques [MESH] |*Transcriptional Activation [MESH] |Cell Differentiation/genetics [MESH] |HEK293 Cells [MESH] |Humans [MESH] |Induced Pluripotent Stem Cells [MESH] |Neurons/cytology [MESH] |RNA, Guide, CRISPR-Cas Systems [MESH]Highly efficient Cas9-mediated transcriptional programming (epmc).pdf DeepDyve Pubget Overpricing