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10.1038/nrg3899 http://scihub22266oqcxt.onion/10.1038/nrg3899 C4503232!4503232 !25854182     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25854182 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nat+Rev+Genet 2015 ; 16 (5 ): 299-311 Nephropedia Template TP {{tp|p=25854182 |t=2015. High-throughput functional genomics using CRISPR-Cas9 |pdf=|usr=}}{{25854182 }} gab.com Text High-throughput functional genomics using CRISPR-Cas9 JO: Nat Rev Genet http://www.kidney.de/pget.php?&tw=1&pmid=25854182 #FOAvip Forward genetic screens are powerful tools for the discovery and functional annotation of genetic elements. Recently, the RNA-guided CRISPR (clustered regularly interspaced short palindromic repeat)-associated Cas9 nuclease has been combined with genome-scale guide RNA libraries for unbiased, phenotypic screening. In this Review, we describe recent advances using Cas9 for genome-scale screens, including knockout approaches that inactivate genomic loci and strategies that modulate transcriptional activity. We discuss practical aspects of screen design, provide comparisons with RNA interference (RNAi) screening, and outline future applications and challenges. Twit Text FOAVip High-throughput functional genomics using CRISPR-Cas9 ????Nat Rev Genet http://www.kidney.de/pget.php?&tw=1&pmid=25854182 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25854182 #FOAvip English Wikipedia <ref>{{Cite pmid|25854182 }}</ref> High-throughput functional genomics using CRISPR-Cas9 #MMPMID25854182 Shalem O ; Sanjana NE ; Zhang F Nat Rev Genet 2015[May]; 16 (5 ): 299-311 PMID25854182 show ga Forward genetic screens are powerful tools for the discovery and functional annotation of genetic elements. Recently, the RNA-guided CRISPR (clustered regularly interspaced short palindromic repeat)-associated Cas9 nuclease has been combined with genome-scale guide RNA libraries for unbiased, phenotypic screening. In this Review, we describe recent advances using Cas9 for genome-scale screens, including knockout approaches that inactivate genomic loci and strategies that modulate transcriptional activity. We discuss practical aspects of screen design, provide comparisons with RNA interference (RNAi) screening, and outline future applications and challenges. |*CRISPR-Cas Systems [MESH] |Animals [MESH] |Gene Knockout Techniques [MESH] |Gene Library [MESH] |Genetic Testing/methods [MESH] |Genomics/*methods [MESH] |High-Throughput Nucleotide Sequencing/methods [MESH] |Humans [MESH] |Mice [MESH] |Models, Genetic [MESH]High-throughput functional genomics using CRISPR-Cas9 (epmc).pdf DeepDyve Pubget Overpricing