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10.1038/nmeth.3486 http://scihub22266oqcxt.onion/10.1038/nmeth.3486 C4589471!4589471!26258292     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26258292.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nat+Methods 2015 ; 12 (10): 989-94 Nephropedia Template TP {{tp|p=26258292|t=2015. High-Throughput, Data-Rich Cellular RNA Device Engineering |pdf=|usr=}}{{26258292}} gab.com Text High-Throughput, Data-Rich Cellular RNA Device Engineering JO: Nat Methods http://www.kidney.de/pget.php?&tw=1&pmid=26258292 #FOAvip Methods for rapidly assessing sequence-structure-function landscapes and developing conditional gene-regulatory devices are critical to our ability to manipulate and interface with biology. We describe a framework for engineering RNA devices from preexisting aptamers that exhibit ligand-responsive ribozyme tertiary interactions. Our methodology utilizes cell sorting, high-throughput sequencing, and statistical data analyses to enable parallel measurements of the activities of hundreds of thousands of sequences from RNA device libraries in the absence and presence of ligands. Our tertiary interaction RNA devices exhibit improved performance in terms of gene silencing, activation ratio, and ligand sensitivity as compared to optimized RNA devices that rely on secondary structure changes. We apply our method to building biosensors for diverse ligands and determine consensus sequences that enable ligand-responsive tertiary interactions. These methods advance our ability to develop broadly applicable genetic tools and to elucidate understanding of the underlying sequence-structure-function relationships that empower rational design of complex biomolecules. Twit Text FOAVip High-Throughput, Data-Rich Cellular RNA Device Engineering ????Nat Methods http://www.kidney.de/pget.php?&tw=1&pmid=26258292 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26258292 #FOAvip English Wikipedia <ref>{{Cite pmid|26258292}}</ref> High-Throughput, Data-Rich Cellular RNA Device Engineering #MMPMID26258292 Townshend B; Kennedy AB; Xiang JS; Smolke CD Nat Methods 2015[Oct]; 12 (10): 989-94 PMID26258292show ga Methods for rapidly assessing sequence-structure-function landscapes and developing conditional gene-regulatory devices are critical to our ability to manipulate and interface with biology. We describe a framework for engineering RNA devices from preexisting aptamers that exhibit ligand-responsive ribozyme tertiary interactions. Our methodology utilizes cell sorting, high-throughput sequencing, and statistical data analyses to enable parallel measurements of the activities of hundreds of thousands of sequences from RNA device libraries in the absence and presence of ligands. Our tertiary interaction RNA devices exhibit improved performance in terms of gene silencing, activation ratio, and ligand sensitivity as compared to optimized RNA devices that rely on secondary structure changes. We apply our method to building biosensors for diverse ligands and determine consensus sequences that enable ligand-responsive tertiary interactions. These methods advance our ability to develop broadly applicable genetic tools and to elucidate understanding of the underlying sequence-structure-function relationships that empower rational design of complex biomolecules. äHigh-Throughput, Data-Rich Cellular RNA Device Engineering (epmc).pdf DeepDyve Pubget Overpricing