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10.1038/nmeth.3486 http://scihub22266oqcxt.onion/10.1038/nmeth.3486 C4589471!4589471 !26258292     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26258292 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26258292 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nat+Methods 2015 ; 12 (10 ): 989-94 Nephropedia Template TP {{tp|p=26258292 |t=2015. High-throughput cellular RNA device engineering |pdf=|usr=}}{{26258292 }} gab.com Text High-throughput cellular RNA device engineering JO: Nat Methods http://www.kidney.de/pget.php?&tw=1&pmid=26258292 #FOAvip Methods for rapidly assessing sequence-structure-function landscapes and developing conditional gene-regulatory devices are critical to our ability to manipulate and interface with biology. We describe a framework for engineering RNA devices from preexisting aptamers that exhibit ligand-responsive ribozyme tertiary interactions. Our methodology utilizes cell sorting, high-throughput sequencing and statistical data analyses to enable parallel measurements of the activities of hundreds of thousands of sequences from RNA device libraries in the absence and presence of ligands. Our tertiary-interaction RNA devices performed better in terms of gene silencing, activation ratio and ligand sensitivity than optimized RNA devices that rely on secondary-structure changes. We applied our method to build biosensors for diverse ligands and determine consensus sequences that enable ligand-responsive tertiary interactions. These methods advance our ability to develop broadly applicable genetic tools and to elucidate the underlying sequence-structure-function relationships that empower rational design of complex biomolecules. Twit Text FOAVip High-throughput cellular RNA device engineering ????Nat Methods http://www.kidney.de/pget.php?&tw=1&pmid=26258292 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26258292 #FOAvip English Wikipedia <ref>{{Cite pmid|26258292 }}</ref> High-throughput cellular RNA device engineering #MMPMID26258292 Townshend B ; Kennedy AB ; Xiang JS ; Smolke CD Nat Methods 2015[Oct]; 12 (10 ): 989-94 PMID26258292 show ga Methods for rapidly assessing sequence-structure-function landscapes and developing conditional gene-regulatory devices are critical to our ability to manipulate and interface with biology. We describe a framework for engineering RNA devices from preexisting aptamers that exhibit ligand-responsive ribozyme tertiary interactions. Our methodology utilizes cell sorting, high-throughput sequencing and statistical data analyses to enable parallel measurements of the activities of hundreds of thousands of sequences from RNA device libraries in the absence and presence of ligands. Our tertiary-interaction RNA devices performed better in terms of gene silencing, activation ratio and ligand sensitivity than optimized RNA devices that rely on secondary-structure changes. We applied our method to build biosensors for diverse ligands and determine consensus sequences that enable ligand-responsive tertiary interactions. These methods advance our ability to develop broadly applicable genetic tools and to elucidate the underlying sequence-structure-function relationships that empower rational design of complex biomolecules. |Aptamers, Nucleotide/*chemistry/metabolism [MESH] |Biosensing Techniques [MESH] |Data Interpretation, Statistical [MESH] |Flow Cytometry/methods [MESH] |Gene Expression Regulation/drug effects [MESH] |Gene Library [MESH] |Genetic Engineering/*methods [MESH] |Green Fluorescent Proteins/genetics [MESH] |High-Throughput Nucleotide Sequencing/methods [MESH] |Ligands [MESH] |Nepovirus/genetics [MESH] |RNA, Catalytic/chemistry [MESH] |Riboswitch/*genetics [MESH] |Surface Plasmon Resonance [MESH]High-throughput cellular RNA device engineering (epmc).pdf DeepDyve Pubget Overpricing