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10.1038/nmeth.3365 http://scihub22266oqcxt.onion/10.1038/nmeth.3365 C4573404!4573404 !25915120     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25915120 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nat+Methods 2015 ; 12 (6 ): 568-76 Nephropedia Template TP {{tp|p=25915120 |t=2015. High-performance probes for light and electron microscopy |pdf=|usr=}}{{25915120 }} gab.com Text High-performance probes for light and electron microscopy JO: Nat Methods http://www.kidney.de/pget.php?&tw=1&pmid=25915120 #FOAvip We describe an engineered family of highly antigenic molecules based on GFP-like fluorescent proteins. These molecules contain numerous copies of peptide epitopes and simultaneously bind IgG antibodies at each location. These 'spaghetti monster' fluorescent proteins (smFPs) distributed well in neurons, notably into small dendrites, spines and axons. smFP immunolabeling localized weakly expressed proteins not well resolved with traditional epitope tags. By varying epitope and scaffold, we generated a diverse family of mutually orthogonal antigens. In cultured neurons and mouse and fly brains, smFP probes allowed robust, orthogonal multicolor visualization of proteins, cell populations and neuropil. smFP variants complement existing tracers and greatly increase the number of simultaneous imaging channels, and they performed well in advanced preparations such as array tomography, super-resolution fluorescence imaging and electron microscopy. In living cells, the probes improved single-molecule image tracking and increased yield for RNA-seq. These probes facilitate new experiments in connectomics, transcriptomics and protein localization. Twit Text FOAVip High-performance probes for light and electron microscopy ????Nat Methods http://www.kidney.de/pget.php?&tw=1&pmid=25915120 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25915120 #FOAvip English Wikipedia <ref>{{Cite pmid|25915120 }}</ref> High-performance probes for light and electron microscopy #MMPMID25915120 Viswanathan S ; Williams ME ; Bloss EB ; Stasevich TJ ; Speer CM ; Nern A ; Pfeiffer BD ; Hooks BM ; Li WP ; English BP ; Tian T ; Henry GL ; Macklin JJ ; Patel R ; Gerfen CR ; Zhuang X ; Wang Y ; Rubin GM ; Looger LL Nat Methods 2015[Jun]; 12 (6 ): 568-76 PMID25915120 show ga We describe an engineered family of highly antigenic molecules based on GFP-like fluorescent proteins. These molecules contain numerous copies of peptide epitopes and simultaneously bind IgG antibodies at each location. These 'spaghetti monster' fluorescent proteins (smFPs) distributed well in neurons, notably into small dendrites, spines and axons. smFP immunolabeling localized weakly expressed proteins not well resolved with traditional epitope tags. By varying epitope and scaffold, we generated a diverse family of mutually orthogonal antigens. In cultured neurons and mouse and fly brains, smFP probes allowed robust, orthogonal multicolor visualization of proteins, cell populations and neuropil. smFP variants complement existing tracers and greatly increase the number of simultaneous imaging channels, and they performed well in advanced preparations such as array tomography, super-resolution fluorescence imaging and electron microscopy. In living cells, the probes improved single-molecule image tracking and increased yield for RNA-seq. These probes facilitate new experiments in connectomics, transcriptomics and protein localization. |Animals [MESH] |Antigens [MESH] |Brain Mapping [MESH] |Drosophila [MESH] |Luminescent Proteins/*chemistry [MESH] |Mice [MESH] |Microscopy, Electron/*methods [MESH] |Microscopy, Fluorescence/*methods [MESH] |Models, Molecular [MESH] |Molecular Sequence Data [MESH] |Neurons [MESH]High-performance probes for light and electron microscopy (epmc).pdf DeepDyve Pubget Overpricing