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Helical buckling of actin inside filopodia generates traction
#MMPMID25535347
Leijnse N
; Oddershede LB
; Bendix PM
Proc Natl Acad Sci U S A
2015[Jan]; 112
(1
): 136-41
PMID25535347
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Cells can interact with their surroundings via filopodia, which are membrane
protrusions that extend beyond the cell body. Filopodia are essential during
dynamic cellular processes like motility, invasion, and cell-cell communication.
Filopodia contain cross-linked actin filaments, attached to the surrounding cell
membrane via protein linkers such as integrins. These actin filaments are thought
to play a pivotal role in force transduction, bending, and rotation. We
investigated whether, and how, actin within filopodia is responsible for
filopodia dynamics by conducting simultaneous force spectroscopy and confocal
imaging of F-actin in membrane protrusions. The actin shaft was observed to
periodically undergo helical coiling and rotational motion, which occurred
simultaneously with retrograde movement of actin inside the filopodium. The cells
were found to retract beads attached to the filopodial tip, and retraction was
found to correlate with rotation and coiling of the actin shaft. These results
suggest a previously unidentified mechanism by which a cell can use rotation of
the filopodial actin shaft to induce coiling and hence axial shortening of the
filopodial actin bundle.
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